长期紫外线照射会引发诸多皮肤相关疾病，皮肤的保养越来越受到学者们的广泛关注。本文研究组方提取物A对大鼠抗光老化的影响。通过病理学分析和电镜检测照射部位皮肤组织病理学形态及超微结构；采用免疫组化和荧光定量PCR检测皮肤基质金属蛋白酶1（MMP-1）、MMP-2、MMP-9和Ia抗原的表达以及皮肤组织中I型胶原、III型胶原、结缔组织生长因子（CTGF）、转化生长因子β1（TGF-β1）基因表达情况。研究表明：与模型对照组相比，组方提取物A低、中、高剂量组照射部位皮肤真皮间质血管扩张、炎细胞浸润、成纤维细胞增生减轻，真皮弹力纤维变性、局灶性蓄积减轻，皮肤表皮厚度、真皮层成纤维细胞数显著降低，照射部位皮肤的超微结构得到改善，提取物A可降低皮肤转化生长因子β1（TGF-β1）的表达，提高皮肤III型胶原的表达。因此，组方提取物A具有较好的抗光老化功效。

Because ultraviolet light (UV) can cause various skin diseases, scientists have been paying increasing attention to the study of skin care. The anti-photoaging effect of an extract A from a traditional Chinese medicine formula was studied on UV-irradiated rats. The morphology and ultrastructure of the skin tissues were determined by pathological analysis and electron microscopy, respectively. Immunohistochemical analysis and the fluorogenic quantitative polymerase chain reaction were used to determine the skin tissue matrix metalloproteinase 1 (MMP-1), MMP-2, MMP-9, and Ia antigen expression levels, as well as the gene expression levels of skin collagen type I and collagen type III, and connective tissue growth factor and transforming growth factor β1 (TGF-β1). Compared with the model control group, rats in the low-, medium- and high-dose extract-A-treated groups showed mitigated dermal interstitial vasodilation, inflammatory cell infiltration, fibroblast hyperplasia, dermal elastic fiber degeneration, and focal accumulation, with decreases in skin thickness of the epidermis and the number of dermal fibroblasts of the irradiated skin. Extract A also improved the ultrastructure of the irradiated skin, and reduced the expression of TGF-β1 while improving that of collagen type III. Therefore, extract A of this traditional Chinese medicine formula possesses strong anti-photoaging activity.

广东省自然科学基金项目（2014A030313242）；中央高校科研业务费项目（2015ZZ110）；广东省科技计划项目（2013B090700008）