本文考察了罗非鱼皮胶原酶解物(TSCH)对双醛淀粉(DAS)交联明胶膜理化性质的影响，探讨了膜对人皮肤永生化角质(HaCat)细胞的相容性。结果发现，TSCH添加量从0增加至16%时，DAS交联明胶膜的抗拉伸强度从55.00 MPa下降至24.85 MPa，而断裂延伸率随着TSCH添加量的增加呈现先增大后减小的趋势。DAS交联明胶膜在30 ℃水中的溶解率为33.10%，当膜中TSCH含量超过4%后，膜的溶解率接近100%。然而，不管TSCH添加量是多少，经过240 min后膜中含有的TSCH全部释放到水中。SDS-PAGE的结果表明，在DAS交联明胶膜中添加的TSCH会阻碍明胶蛋白分子的相互交联作用。另一方面，将HaCat细胞培养于DAS交联明胶膜时，HaCat细胞活力只有11.39%，当DAS交联明胶膜中含有8%的TSCH时，HaCat细胞活力可以达到108.20%。以上结果表明，添加TSCH的DAS交联明胶膜在化妆品领域将具有一定的应用潜力。

The effect of tilapia skin collagen hydrolysate (TSCH) on the physicochemical properties of dialdehyde starch (DAS) cross-linked gelatin films was investigated, and the biocompatibility of these films with the human skin keratinocyte (HaCat) cell was also studied. When the TSCH content in the DAS-gelatin films was increased from 0 to 16%, the tensile strength (TS) of films decreased from 55.00 MPa to 24.85 MPa, while the elongation at break (EAB) showed a downward trend after the initial increase with increasing amount of added TSCH. The solubility of DAS cross-linked gelatin films in water at 30 ℃ was 33.10%, and at TSCH concentration higher than four percent, it increased to 100%. However, TSCH from films was released into water after 240 min irrespective of the amount of added TSCH. The results of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the addition of TSCH in the DAS cross-linked gelatin film could hinder the interaction between the protein molecules of gelatin. On the other hand, when HaCat cell was cultured on the surface of DAS cross-linked gelatin films, the cell activity of HaCat cells was 11.39%. When the DAS cross-linked gelatin film contained 8% TSCH, the HaCat cell activity reached up to 108.2%. Based on the above results, the DAS-gelatin films containing TSCH have potential applications in the cosmetic industry.

国家自然科学基金(31271984)；福建省杰出青年科学基金项目(2014J06013)；厦门市海洋经济发展专项资金(13CZP003HJ05）；闽海渔高新（[2013] 006号）