研究春秋两个季节亮叶杨桐叶中酚类物质体外抗氧化活性及对HepG2细胞的抗增殖作用。采用福林酚法、硼氢化钠/四氯苯醌比色法、超氧自由基吸收能力（ORAC）、超氧自由基清除能力（PSC）、细胞抗氧化法（CAA）和亚甲基蓝染色法分析游离态/结合态多酚含量、游离态/结合态黄酮含量、游离态/结合态提取物胞外抗氧化能力、游离态提取物胞内抗氧化能力和游离态提取物对HepG2细胞的抗增殖作用。秋季亮叶杨桐叶中游离态酚、游离态黄酮含量高，分别是142.69±0.58 mg GAE/g DW和112.98±0.37 CE/g DW；游离态提取物超氧自由基吸收能力强、超氧自由基清除能力强和细胞抗氧化能力强，分别是1723.08±109.27 μmol TE/g DW、24.07±1.98 μM VCE/g DW和900.84±2.68 μmol QE/100 g；游离态提取物对HepG2细胞的抗增殖作用强，EC50值为4.30 mg/mL。亮叶杨桐叶富含酚类物质，特别是秋季亮叶杨桐叶是一种新型的天然抗氧化剂的原料，可进一步开发。

The antioxidant activity of phenolic compounds from Adinandra nitida leaves collected in spring and fall and their antiproliferative effects on HepG2 cells were studied. The free/bound polyphenolic contents and free/bound flavonoid contents in Adinandra nitida leaves were investigated by the Folin reagent method and sodium borohydride/chloranil-based assay (SBC), respectively. The in vitro antioxidant activities of free/bound phytochemicals in the extracts and the in vivo antioxidant activities of free phytochemicals in the extracts were measured through oxygen radical absorbance capacity (ORAC), peroxyl radical scavenging capacity (PSC), and cellular antioxidant activity (CAA) assays. The antiproliferative effect of free phytochemicals from Adinandra nitida leaf extracts was measured by the methylene blue colorimetric method. The free phenolic content and free flavonoid content from autumn Adinandra nitida leaves were high, and the values were 142.69 ? 0.58 mg GAE/g DW and 112.98 ? 0.37 CE/g DW, respectively. The ORAC, PSC, and CAA of free phytochemicals from leaf extracts were relatively strong, and the values were 1723.08 ± 109.27 μmol TE/g DW, 24.07 ± 1.98 μM VCE/g DW, and 900.84 ± 2.68 μmol QE/100 g, respectively. The free phytochemicals from autumn Adinandra nitida leaves showed a strong antiproliferative effect on HepG2 cells, with a half maximal effective concentration (EC50) value of 4.30 mg/mL. Adinandra nitida is rich in phytochemicals, and especially, autumn Adinandra nitida leaves are a new raw material for natural antioxidants.

广东省自然科学基金项目（2014A030313242）；广东省科技计划项目（2013B090700008）；广州市科技计划项目（2013J4500036）；广东省科技计划项目（2012B050500003）；广东省领军人才项目（刘瑞海）；中央高校基本科研业务费专项资金资助(D2155140)