利用液相色谱?四级杆?飞行时间串联质谱（LC-Q-TOF-MS），结合标准品比较、保留时间对比、多级质谱碎片和相关文献，鉴定了月桂叶黄酮的化学成分。利用液相?三重四级杆串联质谱（LC-MS/MS）在多反应监控模式（MRM）下，分别测定了黄酮化合物的含量。从我国月桂叶中分离鉴定出了16种黄酮化合物，包括牡荆素鼠李糖苷（1658.53 μg/g±56.9）、芦丁（19883.54±494.33 μg/g）、牡荆素（4430.87±89.72 μg/g）、A-型原花青素三聚体（2281.88±78.90 μg/g）、表儿茶素（148.10 μg/g±2.32）、山奈酚-3-O-芸香糖苷（3491.02±47.98 μg/g）、异鼠李素-3-O-芸香糖苷（6643.78±99.04 μg/g）、金丝桃苷（11979.66±317.79 μg/g）、山奈酚-7-O-葡萄糖苷（738.55 μg/g±10.86）、紫云英苷（508.60 μg/g±6.96）、槲皮素-3-O-木糖苷（2044.22 μg/g±57.65）、异鼠李素-3-O-葡萄糖苷（2079.94 μg/g±76.38）、槲皮苷（2079.94 μg/g±61.84）、山奈酚-3-O-鼠李糖苷（6412.37±78.65 μg/g）、槲皮素（1629.45 μg/g±47.65）和山奈酚（364.29 μg/g±2.84）。测定了月桂叶黄酮提取物清除DPPH自由基的能力，结果证明，月桂叶黄酮具有良好的清除自由基能力。

Flavonoids in the leaves of Laurus nobilis were identified using liquid chromatography-quadrupole-time of flight mass spectrometry combined with comparison of the retention times and tandem mass spectrometric fragment patterns of standards or from related literature. Flavonoid contents were measured by liquid chromatography-triple quadrupole tandem mass spectrometry in multiple reaction monitoring mode. Sixteen flavonoids were detected in L. nobilis leaves, including vitexin-2-O-rhamnoside (1658.53 ± 56.9 μg/g), rutin (19,883.54 ± 494.33 μg/g), vitexin (4430.87 ± 89.72 μg/g), type-A procyanidin trimer (2281.88 ± 78.90 μg/g), epicatechin (148.10 ± 2.32 μg/g), kaempferol-3-O-rutinoside (3491.02 ± 47.98 μg/g), isorhamnetin-3-O-rutinoside (6643.78 ± 99.04 μg/g), hyperoside (11,979.66 ± 317.79 μg/g), kaempferol-7-glucoside (738.55 ± 10.86 μg/g), astragalin (508.60 ± 6.96 μg/g), quercetin-3-O-xylopyranoside (2044.22 ± 57.65 μg/g), isorhamnetin-3-O-glycosidase (2079.94 ± 76.38 μg/g), quercitrin (2079.94 ± 61.84 μg/g), kaempferol-3-O-rhamnoside (6412.37 ± 78.65 μg/g), quercetin (1629.45 ± 47.65 μg/g), and kaempferol (364.29 ± 2.84 μg/g). The 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities of the extract were determined, and the results clearly demonstrated that flavonoids from L. nobilis possessed significant radical scavenging activities.

天津市自然科学基金重点项目（12JCZDJC34100）