[关键词]
[摘要]
通过建立体外肿瘤坏死因子(TNF-α)诱导人脐静脉血管内皮细胞(HUVEC)炎症损伤反应模型,研究桂花多酚纯化组分1、2、3对炎症细胞的活性(MTT)、活性氧(ROS)含量、超氧化物歧化酶(SOD)和黄嘌呤氧化酶(XOD)活性影响;以10 Ug/L TNF-α诱导HUVEC产生炎症反应,加入6000 Ug/L、3000 Ug/L、300 Ug/L和30 Ug/L桂花多酚纯化组分1、2、3,研究桂花多酚纯化组分1、2、3的浓度及其组成对炎症细胞保护作用的影响。结果表明,当桂花多酚纯化组分2、3浓度为300~3000 Ug/L、组分1浓度为3000 Ug/L时,可显著提高由TNF-α诱导的HUVEC细胞活力和SOD酶活性(p<0.05),显著抑制炎症细胞中活性氧(ROS)含量和XOD酶活性(p<0.05),具有显著抗炎效果。
[Key word]
[Abstract]
An in vitro human umbilical vein endothelial cell (HUVEC) model of inflammation induced by tumor necrosis factor (TNF)-α was established to study the effects of the purified fractions 1, 2, and 3 obtained from the ethanol extracts of Osmanthus fragrans flowers on cell viability (MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay), reactive oxygen species (ROS) content, and the activity of superoxide dismutase (SOD) and xanthine oxidase (XOD) in the inflammatory cells. The inflammatory reaction was triggered by TNF-α (10 μg/L) in HUVECs, and then the inflammatory cells were treated with fractions 1, 2, and 3 at concentrations of 6000, 3000, 300, and 30 μg/L. The protective effects of the concentrations and compositions of fractions 1, 2, and 3 on inflammatory cells were evaluated. The results showed that treatment with fractions 2 and 3 at concentrations ranging from 300–3000 μg/L and fraction 1 at a concentration of 3000 μg/L could significantly enhance HUVEC cell viability and SOD activity (p < 0.05), while significantly suppressing XOD activity and reducing ROS content (p < 0.05). The abovementioned treatment exhibited strong anti-inflammatory effects.
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[基金项目]
江苏省农业科技自主创新资金(CX(14)2120);江苏省自然基金面上项目(BK20141386);江苏省苏北科技专项资金(富民强县)项目(BN2014088);2014年中央财政农业技术推广资金项目(TG(14)113)