为了考察海洋胶原低聚肽中ACE抑制肽的活性和结构，本文以深海鲑鱼皮为原料，采用两步酶解法制备出海洋胶原低聚肽，在对其分子量分布进行分析的基础上，测定了其血管紧张素转化酶（ACE）抑制活性。结果表明，海洋胶原低聚肽中分子量小于1000 u的组分高达90.79%，主要分布在132~576 u范围内，其ACE抑制活性的IC50为1.18±0.12 mg/mL。然后利用反相高效液相色谱（RP-HPLC）对海洋胶原低聚肽进行分离纯化，收集11个组分峰，对其ACE抑制活性进行了测定。结果表明，有7个组分的ACE抑制活性比海洋胶原低聚肽高。最后利用Q-TOF质谱仪对收集的11个组分进行了结构鉴定，并对鉴定出的15个肽段进行了ACE抑制活性测定。结果表明，15个肽段均有一定的ACE抑制活性，其中Ala-Pro（AP）、Val-Arg（VR）、Gly-Arg（GR）的ACE抑制率比海洋胶原低聚肽高，IC50值分别为0.07±0.01 mg/mL、0.35±0.03 mg/mL、0.92±0.85 mg/mL，活性大约是海洋胶原低聚肽的16.86、3.37、1.28倍，是具有较高ACE抑制活性的肽段。

To investigate the activity and structure of angiotensin-converting enzyme (ACE) inhibitory peptides derived from marine collagen oligopeptides (MCOPs), MCOPs were prepared from deep-sea salmon skin by two-step enzymolysis. Based on analysis of the molecular weight distribution, the ACE inhibitory activity of MCOP was determined. The results showed that the components with a molecular weight of less than 1000 u in MCOP accounted for up to 90.79% of all proteins and were mainly distributed in the range of 132~576 u, and the corresponding IC50 for ACE activity was 1.18 ± 0.12 mg/mL. Subsequently, MCOPs were separated and purified by reverse-phase high performance liquid chromatography (RP-HPLC) and the ACE inhibitory activity of 11 fractions was measured. Seven fractions had a higher ACE inhibitory activity than the total MCOP mixture. Finally, structural identification of peptides in the 11 fractions was performed on a quadrupole time-of-flight (Q-TOF) mass spectrometer and the ACE inhibitory activity of the 15 identified peptides was determined. The results indicated that all peptides exhibited ACE inhibitory activity and Ala-Pro (AP), Val-Arg (VR), and Gly-Arg (GR) showed stronger activities than the total MCOP mixture. The IC50 values of the three peptides were 0.07 ± 0.01 mg/mL, 0.35 ± 0.03 mg/mL, and 0.92 ± 0.85 mg/mL, respectively. Their ACE inhibitory activities were 16.86, 3.37, and 1.28 times the inhibitory activity of the MCOP mixture, respectively. Therefore, AP, VR, and GR are peptides with potent ACE inhibitory activity.

国家十二五科技支撑项目（2012BAD33B04-02）；国家高技术研究发展计划（863 计划）项目（2013AA102205-02）；北京市科委科技创新基地培育与发展工程项目（Z121106002812040）；科技北京百名领军人才培养工程项目（Z131110000513026）