本文研究了鲫鱼卵唾液酸糖蛋白（Sialoglycoproteins of Carassius auratus eggs, Ca-SGP）对SAMP6小鼠骨丢失的保护作用。以雄性SAMP6小鼠为老年性骨质疏松症动物模型，连续灌胃Ca-SGP 150d后，检测血清骨代谢生化标志物，测定小鼠股骨矿化沉积率、胫骨骨密度及松质骨微结构。结果显示，经Ca-SGP (500mg/kg?bw)干预后，小鼠血清中骨生成标志物骨源性碱性磷酸酶的活性和I型前胶原羧基端前肽、骨钙素的含量得到显著升高（p<0.01），骨吸收标志物抗酒石酸酸性磷酸酶和组织蛋白酶K的活性得到显著抑制（P<0.01）；胫骨骨密度提高10.92%（p<0.01）；松质骨骨体积分数、骨小梁数目及厚度均得到显著增加（P<0.01），骨小梁间隙显著缩小（P<0.01）；骨矿化沉积率提高98.87%，差异显著（p<0.01）。该研究表明Ca-SGP可改善老年性骨质疏松症小鼠低骨转换，提高骨密度，改善骨小梁结构，促进骨矿化，为开发抗骨质疏松症药物和功能食品提供了理论支持。

The protective effect of Sialoglycoproteins of Carassius auratus eggs (Ca-SGP) on bone loss was examined. The animal model was established by male senescence-accelerated mouse. The mice were intragastric administrated daily with Ca-SGP for 150 days. At the end of the experiment, the bone metabolic indexes, mineral apposition rate (MAR) of femoral, bone mineral density (BMD) and trabecular microstructure of tibia were measured. Results indicated that Ca-SGP (500mg/kg?bw) rescued the decreased bone formation by significantly increasing the activity of bone alkaline phosphatase, propeptide carboxy-terminal procollagen and osteocalein levels in serum (P<0.01). Moreover, it inhibited the over-active bone resorption as evidenced by extremely decreasing the activities of tartrate resistant acid and cathepsin K (P<0.01). Meanwhile, tibial BMD was drastically increased by 10.92% (P<0.01). Ca-SGP enhanced trabecular bone microstructure including bone mass, trabecular number, trabecular thickness, and trabecular separation. In addition, Ca-SGP promoted new bone formation and markedly increased MAR by 98.87% (P<0.01). These results demonstrated that Ca-SGP exhibited ameliorative effect on low bone turnover in aged males with osteoporosis, enhanced their BMD, trabecular microstructure and bone mineralization, which may provide some theoretical basis for the utilization of Ca-SGP as an anti-osteoporotic agent or functional food.

国家自然科学基金（31371816）