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[摘要]
本文运用MTT实验考察姜黄素和右旋龙脑分别在单独作用和联合作用时对黑色素瘤细胞 A375存活率的影响,并进一步运用流式细胞术分析右旋龙脑联合姜黄素抑制黑色素瘤细胞A375增殖的原因。结果表明,姜黄素单独作用对A375细胞增殖有显著的抑制效应,而右旋龙脑单独作用对A375细胞增殖没有表现出明显的抑制作用。当用40 μg/mL右旋龙脑预处理3 h,再与20 μmol/L姜黄素联合作用A375细胞72 h后,其细胞存活率达到最低为10.93%,这比20 μmol/L姜黄素单独处理时细胞的存活率降低了18.27%。进一步的流式结果表明,相比单独姜黄素处理,右旋龙脑与姜黄素联合处理后能明显提高SubG1峰和G2/M比例,右旋龙脑(40 μg/mL)与姜黄素(20 μmol/L)联合作用黑色素瘤细胞A375后,其SubG1和G2/M的含量分别从对照组的 2.0%和 16.4%升高到16.8%和 40.1%,表明右旋龙脑与姜黄素主要是通过诱导细胞凋亡和G2/M阻滞来抑制黑色素瘤细胞 A375 增殖。
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[Abstract]
In the article, MTT assay was applied to investigate the effect of curcumin alone and a separate natural borneol with different concentrations on cell viability of A375 melanoma cell and analysed the natural borneol combined with curcumin inhibit melanoma A375 cell proliferation by flow cytometry. The experiment concluded that curcumin could significantly inhibit the A375 cell proliferation. However, natural borneol was not showed obvious inhibitory effect emphasized on A375 cell proliferation. Melanoma A375 cell was pretreated with 40 μg/mL natural borneol 3 h and combined with 20 μmol/L curcumin 72 h, the cells survival rate reached a minimum of 10.93%, which was decreased by 18.27% compared to 20 μmol/L curcumin alone. Further investigation on the action mechanisms by flow cytometric analysis showed that SubG1 peaks and G2/M phase ratios notably increased when A375 cells were dealt with natural borneol association with curcumin. Natural borneol (40 μg/mL) and curcumin (20 μmol/L) combined effects of melanoma cells A375, which SubG1 and G2/M respectively increase to 16.8% and 40.1% compared with the content of the control group 2.0% and 16.4%, which revealed the inhibition of natural borneol combined with curcumin on A375 cells was related with apoptosis and G2/M cycle arrest to inhibit A375 cell proliferation.
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[基金项目]
广东省自然科学基金面上项目(2014A030313265);广东省扬帆计划项目(201312H05);省部产学研结合项目(2013B020311009,2010A011200019,2011B010600029);华南理工大学中央高校基本科研业务费(2015ZZ050);广东省2015年度省公益研究与能力建设专项资金项目(2015A020209020)资助