醋酸格拉替雷是治疗多发性硬化症的一线药物，可通过诱导调节性CD8+T细胞发挥免疫调节作用。为研究GA诱导的CD8+T淋巴细胞，我们将茶树菇分离的两种凝集素AAL（Agrocybe aegerita lectin）和AAL2（Agrocybe aegerita lectin 2），通过生物素标记结合流式细胞仪，分别分析GA诱导的免疫耐受小鼠模型中脾脏、腹股沟淋巴结CD8+T淋巴细胞的糖基化水平，及细胞因子的表达。发现脾脏AAL+CD8+，AAL2+CD8+T淋巴细胞的比例（%）分别为8.46±3.1和9.08±2.8，淋巴结AAL+CD8+，AAL2+CD8+T淋巴细胞的比例（%）分别为10.65±4.6和8.25±5.9，且脾脏、淋巴结CD8+T淋巴细胞中AAL+细胞的百分比均显著高于CD8-T淋巴细胞 （p<0.05）。淋巴结中AAL+CD8+T细胞表达IFN-γ的细胞比例显著高于AAL-CD8+T细胞， AAL2+CD8+T细胞表达IFN-γ和perforin细胞比例均显著高于AAL2-CD8+T细胞。该结果为CD8+T淋巴细胞表面糖基化研究提供了初步数据，并暗示糖基化水平与细胞因子分泌有关。

Glatiramer acetate (GA) is a first-line agent for the treatment of multiple sclerosis, and it exerts an immunomodulatory function by inducing regulatory CD8+ T cells. In order to investigate GA-induced CD8+ T cells, the lectins AAL (Agrocybe aegerita lectin) and AAL2 were used to analyze the glycosylation level and cytokine expression in CD8+ T lymphocytes in the spleen and inguinal lymph nodes in GA-induced, immune-tolerant mice, by biotin-labeling and flow cytometry. The results showed that the percentages of AAL+CD8+ and AAL2+CD8+ T lymphocytes in the spleen were 8.46 ± 3.1% and 9.08 ± 2.8%, respectively; and the percentages of AAL+CD8+ and AAL2+CD8+ T lymphocytes in the inguinal lymph nodes were 10.65 ± 4.6% and 8.25 ± 5.9%, respectively. Moreover, the percentages of AAL+ cells in CD8+ T lymphocytes in both the spleen and inguinal lymph nodes were significantly higher than those in the CD8- T lymphocytes (p <0.05). In the inguinal lymph nodes, the proportion of AAL+CD8+ T cells expressing IFN-γ was significantly higher than that of AAL-CD8+ T cells, and the proportion of cells expressing IFN-γ+ and perforin in AAL2+CD8+ T cells was also significantly higher than that in AAL2-CD8+ T cells. These studies provide preliminary data for studies on the surface glycosylation of CD8+ T lymphocytes, and indicate that glycosylation level is associated with cytokine secretion.

国家自然科学基金（81102850）；省卫生厅基金（A2011434）；省教育厅育苗工程（LYM11070）；东莞市科技局项目（2011108102049）；湛江市科技攻关项目（2011C3109015）；广东医学院大学生创新实验项目（2013ZYDC004）