本文以39800和19500 u的乌贼硫酸软骨素纯品CS-1，CS-2为原料，探究其保水性、体外抗氧化能力、抑制黑色素瘤生长与分子量之间的关系。其保水性研究表明乌贼硫酸软骨素保水性低于甘油，分子量越大，保水性越强。抗氧化研究表明CS-1、CS-2对DPPH?自由基的半数有效清除浓度IC50分别为：13.64、12.42 mg/mL，纯品的清除率比粗品高，对?OH自由基的半数有效清除浓度IC50分别为：7.52、3.98 mg/mL，清除能力为CS-2>粗品>CS-1，抗氧化能力显著，对Fe3+还原能力的测定间接也表明分子量越小，抗氧化能力越强。抑制黑色素瘤生长的研究表明CS-1和CS-2均对黑色素瘤细胞B16-F10有抑制作用，浓度在120 μg/mL时效果明显，在240 μg/mL浓度时，CS-2的肿瘤抑制率大于CS-1的肿瘤抑制率，分子量越小，抑制肿瘤能力越强，CS-2对肿瘤细胞生长的抑制率已超过半数抑制率，可达到54%，在相同条件下，CDDP的抑制率为79%，可见CS-1，CS-2的抗肿瘤活性很高。

Squid chondroitin sulfate samples CS-1 (molecular weight: 39,800 u) and CS-2 (molecular weight: 19,500 u) were used as the raw materials in this study, and the relationships between molecular weight and moisture retention capacity, antioxidant activity, and ability to inhibit melanoma growth were explored. The results indicated that the moisture retention capability of squid chondroitin sulfate was weaker than that of glycerin, and the higher the molecular weight, the stronger the moisture retention capability. The half-maximal inhibitory concentration (IC50) values of CS-1 and CS-2 in scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH?)?radicals were 13.64 and 12.42 mg/mL, respectively, and the scavenging rate of pure product was higher than that of crude product. The IC50 values of CS-1 and CS-2 in scavenging ?OH radicals were 7.52 and 3.98 mg/mL, respectively, and the scavenging abilities were in the following order: CS-2 > crude > CS-1. Strong antioxidant activity was displayed by the squid chondroitin sulfate. The indirect measurement of the reducing power on Fe3+ also showed that smaller molecular weight resulted in stronger antioxidant activity. The results suggested that both CS-1 and CS-2 could inhibit the growth of B16-F10 melanoma cells, and significant inhibitory effect was seen at a concentration of 120 μg/mL. When the concentration was 240 μg/mL, the tumor inhibition rate of CS-2 was higher than that of CS-1. The smaller the molecular weight, the stronger the antitumor activity. The inhibition rate of tumor cells by CS-2 was higher than the IC50, reaching 54%; under the same conditions, the inhibition rate by cisplatin (CDDP) was 79%, and therefore, CS-1 and CS-2 have significant antitumor activity.

浙江省优先主题计划项目(2009C03017-3)；宁波市自然科学基金项目（2013A610156）；宁波大学“水产”浙江省重中之重学科开放基金项目（xkzsc1523)