[关键词]
[摘要]
血液中糖化血红蛋白(HbA1c)水平是衡量人体内长期血糖状态的标准。国际临床化学和实验医学联合会(IFCC)将高效液相离子交换层析(HPLC)作为检测糖化血红蛋白的标准参考方法;而20 mmol/L NaOH(阴极电解液)和20 mmol/L H2SO4(阳极电解液)形成的稳定pH梯度及含两性电解质的凝胶使毛细管等电聚焦电泳(cIEF)在窄PH梯度下对糖化血红蛋白有着显著的分离效率;本文将对HPLC与凝胶cIEF进行分析比较。采用HPLC和凝胶cIEF方法分别测定随机46例血样样品的HbA1c值,对两组测定结果进行相关性分析。表明两种测定方法相关性良好;但是,由于HPLC方法易受血红蛋白变异体干扰,特异性较低;cIEF不受血红蛋白变异体干扰,特异性高。因此,凝胶cIEF方法可以作为HPLC的替代方法应于糖化血红蛋白的标准检测
[Key word]
[Abstract]
The level of glycated hemoglobin (HbA1c) in the blood is a standard measure of the long-term glycemic status of the human body. High-performance liquid chromatography (HPLC) is the standard reference method for HbA1c measurement as designated by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC). Gel-capillary isoelectric focusing (cIEF) with a stable pH gradient of 20 mmol/L NaOH (catholyte solution) and 20 mmol/L sulfuric acid (anolyte solution) and gels containing ampholytes have outstanding separation power. In this study, HPLC has been compared to gel-cIEF for the measurement of HbA1c. HPLC and gel-cIEF were used to measure the HbA1c values of 46 randomly selected blood samples. Correlation analysis revealed that the two methods showed a good correlation. The HPLC method was prone to interference by hemoglobin variants and exhibited low specificity. The gel-cIEF method was not affected by hemoglobin variants and showed high specificity. In conclusion, gel-cIEF can be used as an alternative method to HPLC for the standard detection of glycated hemoglobin.
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[基金项目]
国家重大科学仪器设备开发专项项目“双向凝胶电泳(2DE)成套设备技术开发与应用的研究”(2011YQ030139)