本文研究了麦胚肽（RVF）对人神经瘤细胞SH-SY5Y损伤的保护作用。采用体外细胞培养法，以人神经瘤细胞SH-SY5Y为材料，建立了饥饿损伤和H2O2损伤模型。结果表明：在40个不同浓度与作用时间的组别中，对SH-SY5Y细胞具有显著营养功能的只有无血清饥饿模型中200 μmol/L RVF作用12 h与含1%血清饥饿模型中500 μmol/L RVF作用24 h两个组别，且不具有剂量与时间依赖性。在H2O2诱导的细胞氧化损伤模型中，200 μmol/L的RVF将细胞存活率分别提高了25.05%（MTT法）和21.90%（SRB法）；200 μmol/L RVF预孵育时间延长至12 h时，RVF 极显著地提高了细胞存活率，且具有剂量与时间依赖性；细胞形态检测结果表明RVF干预后，SH-SY5Y细胞也逐渐由皱缩、细长恢复到梭形或多角形，粘附性增强。在本研究建立的两种损伤模型中，RVF仅对H2O2损伤模型具有显著保护作用。

The protective effects of wheat germ peptide (RVF) on the SH-SY5Y human neuroblastoma cell line were investigated in this study. Starvation- and hydrogen peroxide (H2O2)-induced injury models were established by in vitro cell culturing of an SH-SY5Y human neuroblastoma cell line. The SH-SY5Y cells were treated with 40 RVFs at different concentrations and incubation times. The results showed significant nutritional functions only in the group pre-incubated with 200 μmol/L RVF for 12 h in a no-serum starvation-induced injury model and the group treated with 500 μmol/L RVF for 24 h in the 1% serum starvation-induced injury model, and no dose- or time-dependent relationships were found . In the H2O2-induced injury model, treatment with 200 μmol/L RVF increased cell survival rates by 25.05 and 21.90% in the methyl-tetrazolium (MTT) and sulforhodamine B (SRB) assays, respectively, in a time- and dose-dependent manner. The cell morphological analysis indicated that after RVF treatment, the SH-SY5Y cells gradually resumed a spindle or polygonal shape from a wrinkled, shrunken, and elongated shape, with enhanced cell adhesion. In the two injury models investigated in this study, RVF only showed a significant protective effect in the H2O2-induced injury model.

国家自然科学基金项目（31171627，31071523）;“十二五”国家科技支撑计划（2012BAD34B02）