采用荧光光谱、紫外-可见吸收光谱、傅里叶变换红外光谱和拉曼光谱法，研究模拟生理条件下芳樟醇与牛血清白蛋白 (BSA)之间的相互作用机制及芳樟醇对BSA构象的影响。实验表明,芳樟醇可以有规律地猝灭BSA内源荧光，猝灭机制主要为形成芳樟醇-BSA复合物的静态猝灭。通过计算得出二者在不同温度下的结合常数和结合位点数。根据热力学参数判断芳樟醇与BSA之间的相互作用是一个吉布斯自由能降低的自发过程，主要作用力是氢键和范德华力，同时由Forster’s非辐射能量转移理论求得其结合距离。紫外-可见吸收光谱、同步荧光光谱、傅里叶变换红外光谱和拉曼光谱研究表明芳樟醇与BSA相互作用后使BSA构象发生改变，减少了BSA中α-螺旋的含量，增加了BSA中色氨酸、酪氨酸残基微环境的疏水性。

The mechanisms of the interaction between linalool and bovine serum albumin (BSA) and the effect of linalool on the BSA conformation under simulated physiological conditions were investigated by fluorescence, ultraviolet-visible (UV-Vis) absorption, Fourier transform infrared (FT-IR)and Raman spectroscopies. The obtained results indicate that linalool can quench the intrinsic fluorescence of BSA regularly, and that the mechanism underlying was the static quenching via the was the formation of BSA-linalool complexes. The binding constants and the numbers of binding sites at different temperatures were calculated. According to the thermodynamic parameters, the interaction between linalool and BSA is a spontaneous process with a decrease in the Gibbs free energy value. The main forces in this process are hydrogen bonding and van der Waals forces. The binding distance between linalool and BSA was calculated according to the Forster’s theory of non-radioactive energy transfer. Furthermore, the UV-Vis, synchronous fluorescence, FT-IR and Raman spectra demonstrated that the conformation of BSA changes after its interaction with linalool, which includes a decrease in α-helical content and a more hydrophobic microenvironment of tryptophan and tyrosine residues.

广东省教育部产学研结合重大项目（2012A090300010）；广州市科技计划项目(2014Y2-00107)