采用PC12细胞模型，以加合法为协同评价方法，通过测定LDH、MDA和SOD等指标，研究了番薯提取液与茶多酚、葛根黄酮的协同抗氧化作用。结果表明：对于LDH和MDA，茶多酚、葛根黄酮和番薯提取液各浓度组合中的SE值均小于1，说明各组合均能够对LDH和MDA产生显著抑制作用（p<0.01或p <0.05），对于SOD，各浓度组合中的SE值均大于1，说明各组合均能够对SOD产生显著协同作用（p <0.01或p <0.05）；茶多酚、葛根黄酮和番薯提取液的浓度分别为10 mg/L、50 mg/L、2%时，LDH和MDA的SE值均最小，说明该组合对LDH和MDA的协同抑制最强；茶多酚、葛根黄酮和番薯提取液的浓度分别为5 mg/L、10 mg/L、1%时，SOD的SE值最大，说明该组合对SOD的协同作用最强。茶多酚、葛根黄酮和番薯提取液对PC12细胞的LDH、MDA和SOD均有显著影响（P<0.05），说明茶多酚与葛根黄酮共存于番薯中时，能够与番薯产生显著的协同抗氧化作用。

Using the adding method, the synergistic antioxidant properties of sweet potato extracts (SPE) in combination with tea polyphenols (TP) and pueraria flavonoids (PF) on PC12 cells were studied via determining the malondialdehyde (MDA) content and the activities of lactic dehydrogenase (LDH) and superoxide dismutase (SOD). The results showed that the SE (LDH and MDA) of different combinations of SPE, TP, and PF concentrations was less than one, indicating that the combinations exhibited significant inhibitory effects on LDH and MDA (p < 0.01 or p < 0.05); the SE (SOD) value of all combinations was more than one, indicating that the combinations exhibited significant synergistic effects on SOD (p < 0.01 or p < 0.05). For 10 mg/L TP, 50 mg/L PF, and 2% SPE, SE (LDH and MDA) value was the lowest, indicating that this combination showed the strongest inhibition of LDH and MDA. For 5 mg/L TP, 10 mg/L FP, and 1% SPE, SE (SOD) value was the highest, indicating that this combination showed the strongest synergistic effect on SOD. Thus, the effects of TP, FP, and SPE on LDH, MDA, and SOD were significant (P < 0.05), indicating that TP and FP coexist in sweet potato and can produce significant synergistic antioxidant effects.

广东省科技计划农业公关项目（2012A020602038）