[关键词]
[摘要]
为初步探究青钱柳多糖(CPP)对高脂血症小鼠降血脂作用和机制,将高脂血症小鼠随机分为模型对照组、辛伐他汀组、空白对照组以及青钱柳低、中、高剂量组,每组16组。模型对照组与空白对照组均喂以等量的无菌水,辛伐他汀组喂以4 mg/kg的辛伐他汀与CMC-Na(0.5%)的混悬液,青钱柳低、中、高剂量组分别喂以100 mg/kg、200 mg/kg和400 mg/kg的CPP水溶液,连续喂养4周。实验结束后,测定血清TC、TG、HDL-C、LDL-C、FFA水平和LPS活力,取肝脏做病理形态学观察,用RT-PCR方法测定HSL mRNA的表达量。结果表明,与模型对照组相比,CPP组小鼠血清TC、TG、LDL-C的水平和LPS的活力(P<0.01)均显著降低,HDL-C和FFA的水平(P<0.01)则显著增加;CPP组小鼠肝脂变程度较模型组有不同程度的改善;相比于模型对照组,CPP组的HSL mRNA的表达量均显著上升(P<0.05)。综上,CPP可以明显降低高脂血症小鼠血脂水平,改善其因摄入过多脂质而导致肝脏脂肪变性。
[Key word]
[Abstract]
To preliminarily investigate the hypolipidemic mechanism and effect of Cyclocarya paliurus polysaccharides (CPP) in hyperlipidemic mice, the mice were randomly divided into model control, blank control, simvastatin, low dose CPP, medium dose CPP, and high dose CPP groups (16 mice per group). Model control and blankcontrol groups were orally administered with equivoluminal bacteria-free water. The simvastatin group was administered with a suspension of 4 mg/kg simvastatin and carboxymethylcellulose sodium (CMC-Na; 0.5%). The low-dose, medium-dose, and high-dose CPP groups were administered with 100 mg/kg, 200 mg/kg, and 400 mg/kg CPP water solutions, respectively. After four weeks of feeding, the lipopolysaccharide (LPS) activity and the levels of total cholesterol (TC), triglycerides (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), HDL-C, and free fatty acids (FFA) were determined. The liver was removed to assess the pathological morphology and reverse transcription polymerase chain reaction (RT-PCR) was employed to detect the expression level of hormone-sensitive lipase (Hsl) mRNA. The results indicated that CPP induced a significant reduction in TC, TG, and LDL-C levels and LPS activity (P < 0.01), a significant increase in HDL-C and FFA levels (P < 0.01), and different degrees of improvement in the degree of hepatic steatosis when compared to the model group. Compared with the model group, a remarkable increase of Hsl mRNA expression was observed in CPP groups (P < 0.05). In summary, CPP can significantly decrease blood lipid levels and improve hepatic steatosis due to excessive uptake of lipids in hyperlipidemic mice.
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[基金项目]
国家自然科学基金资助项目(31160319);江西省高等学校科技落地计划资助项目(KJLD13027);江西省教育厅科技计划资助项目(GJJ13281);研究生创新项目(YC2014-S188)