对添加茶树菇多糖的益生菌酸奶的体外抗氧化活性及D-半乳糖致衰老小鼠抗衰老效果进行探索，为茶树菇多糖酸奶的抗衰老机制提供依据。结果表明：茶树菇多糖酸奶可有效清除羟自由基、DPPH和ABTS自由基，清除能力在一定浓度范围内与多糖添加量呈正相关；茶树菇多糖的益生菌酸奶可显著提高衰老小鼠血清及不同组织中SOD、CAT、GSH-PX活性，降低MDA含量（P<0.05），且以高剂量组（1200 mg/kg?d）效果最优。高剂量组分别与衰老组和酸奶组相比，小鼠血清、肝脏、脑组织中SOD活力提高了55.87%、79.09%、58.08%和27.48%、15.52%、22.36%；血清、肝脏中GSH-PX活力提高60.86%、41.37%和21.07%、21.63%；血清、肝脏、脑中MDA含量降低78.75%、65.48%、44.93%和69.34%、46.23%、31.77%；脑、肝脏中CAT活力提高76.51%、57.21%和50.15%、33.66%。结论：茶树菇多糖酸奶可发挥茶树菇多糖和益生菌酸奶在清除自由基的体外抗氧化能力及小鼠抗衰老功能的协同作用。

The in vitro antioxidant activities and anti-aging effects of probiotic yogurt incorporated with Agrocybe aegerita polysaccharides in D-galactose-induced aging mouse model were determined to provide a basis for the anti-aging mechanism of A. aegerita polysaccharide yogurt. The results showed that the A. aegerita polysaccharide yogurt could effectively remove hydroxyl free radicals, 1,1 -Diphenyl- 2-picrylhydrazyl radical 2,2- Diphenyl -1- (2,4,6-trinitrophenyl) hydrazyl (DPPH) and 2,2′- azino-bis (3- ethylbenzo- thiazoline-6 -sulfonic acid diammonium salt (ABTS) free radicals, and their scavenging capabilities were positively correlated with the amount of added polysaccharides within a certain concentration range. A. aegerita polysaccharide probiotic yogurt also significantly increased the activities of SOD, CAT, and GSH-PX and reduced MDA content in the aging mouse model in different tissues and serum (P < 0.05), and the high-dose group (1200 mg/kg/d) had the strongest effect. Compared with the aging model group and the yogurt group, the SOD activity of high dose group in the serum, liver, and brain tissues increased by 55.87%, 79.09%, and 58.08%, and 27.48%, 15.52%, and 22.36%, respectively. The serum and liver GSH-PX activity increased by 60.86% and 41.37%, and 21.07% and 21.63%, respectively. The MDA content in the serum, liver, and brain tissues decreased by 78.75%, 65.48%, and 44.93%, and 65.48%, 46.23% and 31.77%, respectively. The CAT activity in the brain and the liver increased by 76.51% and 57.21%, and 50.15% and 33.66%, respectively. Conclusion: A. aegerita polysaccharide yogurt possesses the synergistic in vitro antioxidative capacity via scavenging free radicals and anti-aging function in mice consuming A. aegerita polysaccharides and probiotic yogurt.