[关键词]
[摘要]
本研究对红米麸皮中多酚进行了提取,并通过筛选大孔树脂及对应洗脱剂浓度确定了纯化红米多酚的条件,鉴定了红米多酚的主要成分,探究了红米多酚对碳水化合物在小肠消化吸收过程中涉及的消化酶活性以及葡萄糖吸收的影响。结果表明,大孔树脂HPD400A对红米多酚具有较高的吸附率与解吸率,乙醇浓度70%可达到红米多酚解吸率0.97。红米多酚的主要成分为原花青素。红米多酚对α-淀粉酶、α-葡萄糖苷酶、麦芽糖酶、蔗糖酶活性均有抑制作用,红米多酚浓度越高,抑制作用越强。红米多酚对α-淀粉酶、α-葡萄糖苷酶、蔗糖酶、乳糖酶活性抑制的IC50分别为3.61 μg/mL、2.81 mg/mL、5.48 mg/mL、6.55 mg/mL。红米多酚对离体小肠葡萄糖吸收同样存在抑制作用,红米多酚浓度越高,抑制作用越强,浓度为2.01 mg/mL时红米多酚对葡萄糖吸收的抑制率达72.32%。
[Key word]
[Abstract]
The extraction of polyphenols from red rice bran was investigated and the purification conditions of red rice polyphenols were established by selecting macroporous resin and eluent concentration. Additionally, the major components of red rice polyphenols were identified, and the effect of red rice polyphenol on the related digestive enzyme activities and glucose adsorption during in vitro small intestinal digestionas well as adsorption of carbohydrate was evaluated. The results showed that the adsorption and desorption rates of red rice polyphenol were relatively high when using macroporous resin HPD400A, and the desorption rate of red rice polyphenol could reach 0.97 with ethanol at a concentration of 70%. The main contents of red rice polyphenol are proanthocyanidins. Red rice polyphenol could inhibit the activities of α-amylases, α-glucosidase, maltase, and sucrase, with 50% inhibitory concentration (IC50) values of 3.61 μg/mL, 2.81 mg/mL, 5.48 mg/mL, and 6.55 mg/mL, respectively. Furthermore, the higher the concentration of red rice polyphenols was, the stronger the inhibitory effect became. Additionally, red rice polyphenols could inhibit in vitro intestinal glucose uptake; the higher the concentration of red rice polyphenols was, the stronger the inhibitory effect became. At the concentration of 2.01 mg/mL, the inhibition rate of red rice polyphenols against glucose uptake was 72.32%.
[中图分类号]
[基金项目]
“十二五”国家科技支撑计划课题(2012BAD34B08)