[关键词]
[摘要]
为探讨紫贻贝体内重金属Cd残留的脱除方法,采用海水中添加制备的蛋白水解肽-Fe2+配合物(TPH-Fe2+)进行暂养,检测贻贝不同组织器官中Cd含量变化情况。结果显示,贻贝不同器官中Cd富集量,依次为内脏团>鳃>后闭壳肌>外套膜>前闭壳肌,且在0~10 d自然净化过程中,无显著性下降趋势(P>0.05)。添加10 mg/L TPH-Fe2+净化处理,对贻贝内脏团及鳃中Cd含量无显著性影响;添加15~25 mg/L TPH-Fe2+净化处理贻贝4 d后,内脏团、鳃、前后闭壳肌及外套膜中Cd含量均有不同程度下降(P<0.05);20 mg/L TPH-Fe2+净化10 d后,贻贝不同器官中Cd脱除率表现为鳃>内脏团>外套膜>前后闭壳肌,最高脱除率分别为41.00%、37.43%、29.81%和29.01%,与25 mg/L TPH-Fe2+净化处理组无显著性差异(P>0.05)。研究表明,蛋白水解肽-Fe2+配合物有效脱除贻贝体内Cd残留,可作为一种双壳贝类养殖饲料添加剂加以应用。
[Key word]
[Abstract]
In order to determine an efficient method for the removal of residual cadmium (Cd) from mussel (Mytilus edulis) tissues, mussels were temporarily raised in seawater containing Trichiurus haumela protein hydrolysate-Fe2+ complex (TPH-Fe2+), and the changes in the Cd content of different tissues and organs were measured. The results indicated that the amounts of accumulated Cd in different mussel organs were in the following order: visceral mass > gill > posterior adductor muscle > mantle > anterior adductor muscle; no obvious change of the Cd content in mussel (Mytilus edulis) tissues was observed (P > 0.05) during the 10-day natural depuration period. After the ten-day treatment with 10 mg/L of TPH-Fe2+, there were no significant changes in the Cd concentrations in the visceral mass and gill of mussel. After a four-day treatment with 15~25 mg/L of TPH-Fe2+, the Cd content in the visceral mass, gill, posterior adductor muscle, mantle, and anterior adductor muscle decreased to various degrees (P < 0.05). After a ten-day treatment with 20 mg/L of TPH-Fe2+, The highest removal rates were 41.00%, 37.43%, 29.81%, and 29.01% for gill, visceral mass, mantle, posterior/anterior adductor muscle, respectively. Similar results were observed after a ten-day treatment with 25 mg/L of TPH-Fe2+ (P > 0.05). The results indicated that TPH-Fe2+ can effectively remove residual Cd in mussels and can be used as an aquaculture feed additive for bivalve mollusks.
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[基金项目]
国家青年自然科学基金项目(31201452);浙江省自然科学基金项目(LY15C200017);舟山市科技计划项目(2013C41015)