本文以牛乳铁蛋白素LfcinB和人乳铁蛋白素LfcinH为研究对象，研究其对小鼠脾淋巴细胞增殖及分泌细胞因子的影响并比较两者的差异及相关性。采用CCK-8法检测不同浓度的Lfcin单独作用对小鼠脾淋巴细胞增殖的影响及不同质量浓度Lfcin与丝裂原共同作用对小鼠T、B淋巴细胞增殖作用的影响；ELISA法测定IL-2、IL-4和TNF-α的含量。结果显示：LfcinB和LfcinH能显著促进脾淋巴细胞增殖及增强Con A诱导的T淋巴细胞的增殖和LPS诱导的B淋巴细胞的增殖，并且能显著促进脾淋巴细胞分泌IL-2、IL-4、TNF-α (P<0.01)。研究结果表明：Lfcin能有效的促进脾淋巴细胞的增殖，并与Con A、LPS对T、B淋巴细胞的增殖有协同刺激作用，其机制可能是通过促进细胞因子的分泌发挥免疫增强作用。

The effects of human lactoferricin (LfcinH) and bovine lactoferricin (LfcinB) on the proliferation and cytokine secretion of spleen lymphocytes in mice were studied, and the differences and correlation between LfcinH and LfcinB were compared and analyzed, respectively. The cell counting kit-8 (CCK-8) assay was used to measure the effects of different concentrations of LfcinH or LfcinB alone on the proliferation of mouse spleen lymphocytes and of different concentrations of LfcinH or LfcinB combined with a mitogen on the proliferation of mouse T and B lymphocytes. The IL-2, IL-4, TNF-α content was determined by enzyme-linked immunosorbent assay (ELISA). The results showed that LfcinH and LfcinB could significantly promote the proliferation of spleen lymphocytes and enhance the T lymphocyte proliferation induced by concanavalin A (ConA) and B lymphocyte proliferation induced by lipopolysaccharide (LPS). Moreover, Lfcin could significantly increase the secretions of IL-2, IL-4, TNF-α in spleen lymphocytes. The results also showed that Lfcin has a synergistic effect on the proliferation of T and B spleen lymphocytes with Con A or LPS; the mechanism may be related to the immune enhancement achieved by promoting cytokine secretion.

“十二五”国家科技支撑计划项目（2013BAD18B00）