采用碱溶酸沉法获得长白山核桃楸种仁分离蛋白，利用胰蛋白酶水解后经柱层析分离并通过Shotgun蛋白质组学方法进行鉴定，共识别出61个蛋白质。基于Gene Ontology进行分类显示，61个蛋白质分子量分布在1~100 kDa之间，其中21个蛋白分子量分布在10~20 kDa之间；19个蛋白质（31.15%）等电点分布在pI 6.00~7.00；28个蛋白残基数目在100~300之间；49个蛋白GRAVY值小于0.00，为亲水性蛋白；10个蛋白有跨膜区；这61个蛋白质按生物途径可分为13类，共有42个蛋白质参与代谢过程；按照细胞定位分类，有24个蛋白质来源于细胞，16个蛋白质来源于细胞器；按其分子功能可分为6类，具有催化活性的蛋白质34个，占55.74%，其次是具有结合能力的蛋白质26个，占42.62%。本研究通过核桃种仁蛋白质谱分析，为研究核桃蛋白对机体的作用提供了理论基础和实验数据。

Juglans mandshurica Maxim kernel protein isolates, prepared by alkaline solubilization and acid precipitatio, were hydrolyzed with trypsin, isolated through column chromatography, and identified using shotgun proteomics. From the hydrolyzate, 61 proteins were identified. Gene Ontology classification showed that these proteins were mainly distributed in a range of 1~100 kDa. Among them, 21 proteins were in a range of 10~20 kDa and the isoelectronic points (pI) of 19 proteins (31.15%) were between 6.00~7.00. The number of residues ranged from 100~300 in 28 proteins. The 49 proteins had GRAVY values <0.00, being of hydrophilic proteins. 10 proteins had transmembrane domains. Walnut proteins could be divided into 13 categories by biological processes, and 42 proteins were related to metabolic processes. Regarding the cellular components, 24 proteins were from cells and 16 were from organelles. Regarding molecular function, the proteins could be classified into six types; 34 proteins (55.74%) exhibited catalytic activities, and 26 proteins (42.62%) possessed binding activities. Through mass spectrometry, the present study provided a theoretical basis and experimental data for the functions of walnut kernel proteins in the body.

国家“863”计划项目（2013AA102206）