本研究以活刺参为原料，经紫外线（1.5 W/m2）照射不同时间后，检测刺参体壁和肠中的组织蛋白酶L（CL）、乙酰胆碱酯酶（AChE）、蛋白质羰基、过氧化氢（H2O2）、caspase-3、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)的活力变化和酸性谷胱甘肽(GSH)的变化。研究结果表明，活刺参经UV照射6 h，体壁和肠中CL活力提高了69.27%、52.37%，AChE活力下降了55.70%、36.16%，蛋白质羰基量都是对照组的1.30倍左右，H2O2量提高了2.44倍、3.57倍，Caspase-3活力提高了4.09倍、2.81倍，而体壁和肠中SOD、CAT、GPx的酶活随着UV照射时间的延长而降低,分别为38.79%、24.70%，44.27%、36.18%和79.54%、33.79%，GSH含量增加75.31%、66.71%，说明刺参在UV照射下在发生自溶的同时伴随了氧化损伤和细胞凋亡现象，UV诱导的自溶途径中ROS以及相关氧化产物对于刺参自溶起到了作用，研究结果对于丰富海参自溶机理具有一定的意义。

Live sea cucumbers (Stichopus japonicus) were irradiated under ultraviolet (UV) radiation (1.5 W/m2) for different times. Cathepsin L (CL), acetylcholinesterase (AChE), caspase-3, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), acid glutathione (GSH), protein carbonyl, and hydrogen peroxide (H2O2) contents in the intestinal tissue and body wall of the sea cucumber were analyzed. The results showed that after 6-h UV irradiation, the CL activities, H2O2 contents, and caspase-3 activities in the body wall and intestinal tissue increased by 69.27 and 52.37%, 2.44- and 3.57-fold, and 4.09- and 2.81-fold, respectively. The AChE decreased by 55.70 and 36.16%, respectively and the protein carbonyl contents in both body wall and intestinal tissue was 1.30-fold that of the control group. With increasing UV radiation time, SOD, CAT, and GPx activities in the body wall and intestinal tissue decreased by 38.79 and 24.70%, 44.27 and 36.18%, and 79.54 and 33.79%, respectively. The GSH contents increased by 75.31 and 66.71%, respectively. The results indicate that oxidative damage and apoptosis occurred with autolysis in S. japonicus under UV irradiation. ROS and related oxidation products played a fundamental role in the UV-induced autolysis pathway. This finding may improve the understanding of autolysis in S. japonicus.

国家自然科学基金资助项目（31201299）