利用超滤膜对鲍鱼内脏酶解物进行分离，考察了各分子量段酶解物的成分组成和抗氧化活性。利用截留分子量为10000 u、3000 u、500 u的超滤膜分离得到FA、FB、FC和FD等四个组分中，FA组分的总糖和蛋白含量分别为34.06%和17.20%，而FB、FC和FD等组分主要是由蛋白组成，其游离氨基酸含量分别高达23.20、25.4、28.68 g/100g。根据Tricine-SDS-PAGE和β-消除反应的分析结果，表明FA中位于间隔胶的高分子组分(HMWF)含有N-糖苷键结合的糖蛋白，而分子量为25 ku的成分主要是蛋白。凝胶渗透色谱数据表明FB、FC和FD的分子量主要分布在300 u左右。另一方面，体外抗氧化结果表明，在低浓度下FA组分具有较强的DPPH自由基清除活性和还原力；当质量浓度高于5 mg/mL时，各超滤组分的DPPH自由基清除活性无明显差异，但FB、FC和FD组分的还原力明显高于FA组分；不管是低浓度还是高浓度，FB、FC和FD组分的羟自由基清除活性都明显高于FA组分。

Abalone viscera hydrolysates were separated by ultrafiltration, and the chemical composition and antioxidant activity of the hydrolysates in different ranges of molecular-weight were investigated. Four fractions (FA, FB, FC, and FD) were obtained by membrane ultrafiltration with molecular weight cut-off (MWCO) of 10000, 3000, and 500 u. In fraction FA, total sugar and protein contents were 34.06% and 17.20%, respectively. However, fractions FB, FC, and FD were mainly composed of proteins, and the free amino acid content was 23.20, 25.47, and 28.68 g/10 g, respectively. Based on the results from tricine-sodium dodecyl sulfate polyacrylamide gel electrophoresis and β-elimination, the high molecular-weight fraction (HMWF) of FA in the spacer gel contained glycoproteins with N-glycosidic linkage, and components with molecular weight of 25 ku were proteins. Gel permeation chromatography data indicated that molecular weight of the fractions FB, FC, and FD were each approximately 300 u. Additionally, the result of in vitro antioxidant assay indicated that fraction FA had a relatively strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging ability and reducing power at low concentration. When the concentration was increased above 5 mg/mL, there were no significant differences in the DPPH radical-scavenging abilities among these fractions, but the reducing power of fraction FA was lower than that of other fractions. In both, low and high concentrations, the hydroxyl radical-scavenging abilities of fractions FB, FC, and FD were stronger than that of FA.

厦门市海洋经济发展专项资金(13CZP003HJ05）；海洋公益性行业科研专项（201405016）