[关键词]
[摘要]
本文考察了鲨鱼皮胶原酶解物(SH)、罗非鱼皮胶原酶解物(TH)及其分离组分对人皮肤角质细胞(HaCat细胞)生长的影响。结果发现,在细胞生长对数期阶段,添加0.05 mg/mL或0.20 mg/mL的SH可以明显促进HaCat细胞增殖,然而当SH的浓度提高至1.00 mg/mL时HaCat细胞却没有出现显著的增殖。虽然TH也可以促进HaCat细胞增殖,但TH浓度对细胞增殖却没有显著的影响。另一方面,不管是鲨鱼皮胶原酶解物还是罗非鱼皮胶原酶解物,利用Sephadex G-15凝胶层析分离获得的组分比单组分Gly-Tyr二肽和酪氨酸具有更好的促进细胞增殖能力,在抑制细胞的MDA生成和提高细胞的SOD活力甚至优于谷胱甘肽。这些结果表明,鱼皮胶原制备的酶解物通过凝胶层析分离的组分拥有优越的促进人皮肤角质细胞生长的能力,作为抗氧化肽具有替代谷胱甘肽应用在化妆品的潜力。
[Key word]
[Abstract]
This study investigated the effects of shark skin collagen hydrolysates (SH), tilapia skin collagen hydrolysates (TH), and their isolated fractions on the viability of human skin keratinocyte (HaCat). The HaCat cell viability, determined with the MTT method, was increased notably with the addition of 0.05 mg/mL or 0.20 mg/mL SH during the exponential growth phase. However, when the concentration of the added SH was increased to 1.00 mg/mL, no significant proliferation of HaCat was observed. The HaCat cell viability with the addition of TH was higher than that of the control (without the addition of TH), but TH concentrations did not significantly influence the viability. On the other hand, the HaCat cell viability with addition of the fractions, which were isolated from SH or TH by Sephadex G-15, was much higher than that with the addition of dipeptide (Gly-Tyr) or Tyr. Compared to the sample with added GSH, the MDA content was lower and the SOD activity was higher with the added isolated fractions irrespective of fish skin collagen hydrolysates. Based on these results, it was concluded that the fractions isolated from fish skin collagen hydrolysates by Sephadex G-15, can effectively promote the proliferation of HaCat and have great potential to replace GSH as antioxidative peptides in the cosmetics industry.
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[基金项目]
厦门市科技计划项目(3502Z20131152);福建省杰出青年科学基金项目(2014J06013);厦门市海洋经济发展专项资金(13CZP003HJ05)