研究近江牡蛎糖胺聚糖体内外抗肿瘤活性。采用MTT法测定近江牡蛎糖胺聚糖（CG）对K562、CNE-2Z、Hela细胞增殖的影响；采用动物体内移植性肿瘤模型，观察CG对S180实体瘤和艾氏癌腹水型（EAC）、白血病L1210腹水瘤的影响。实验显示近江牡蛎糖胺聚糖级分CG、CGIa和CGIIa均可以抑制K562、CNE-2Z、Hela细胞的生长（p<0.05），其中CGIa对肿瘤细胞增殖的抑制作用最强，72 h时对K562、CNE-2Z细胞增殖抑制作用的IC50分别为1.95、7.36 mg/L；且对抗肿瘤药物5-Fu具有增敏作用。CG对移植性 S180 肉瘤抑瘤率可达到44.66%，与CTX合用后可提高至67.96%，且对CTX损伤的小鼠免疫功能有一定的修复作用；CG与CTX合用后能延长L1210腹水瘤小鼠和荷EAC小鼠的生命，其生命延长率分别高达50.27%和55.27%。表明近江牡蛎糖胺聚糖具有体内外抗肿瘤活性，其作用可能与直接细胞毒作用及增强机体免疫功能有关。

The anti-tumor activity of glycosaminoglycan from Crassostrea rivularis (CG) in vivo and in vitro was studied in this paper. MTT method was used to detect the effect of CG on the growth of K562, CNE-2Z and Hela cells in vitro. Through the method of animal transplant tumor screening in vivo, the effects of CG on the growth of S180, EAC and L 1210 tumors in mice were observed. The results showed that the fractions of CG, CGIa and CGIIa could inhibit the growth of K562, CNE-2Z and Hela cells (p<0.05), and when combined with 5-Fu the efficiency of anti-tumor increased. IC50 of CGIa on K562 and CNE-2Z cells in 72h were 1.95 mg/L and 7.36 mg/L respectively. In vivo, CG had remarkable inhibitory effect on growth of S180 tumor. The inhibitory ratio increased from 44.66% to 67.96% when combination with CTX. The immune injury by CTX could be repaired by CG. The life prolonging rate of mice transplanted with EAC and L1210 tumor while treated by CG combination with CTX were 50.27% and 55.27%. The results showed that glycosaminoglycan from Crassostrea rivularis had anti-tumor activity.

广东省海洋渔业科技推广专项(A201008I02)