本文探究了山腊梅叶提取物对小鼠脂肪的干预作用及其可能的作用机制。体外脂肪合酶抑制剂的筛选实验结果表明山腊梅叶的醇提物和挥发油对该酶有抑制作用。在体内试验中，将小鼠分为正常组，能量限制组，醇提高剂量组，醇提低剂量组以及挥发油组，每隔两天记录一次体重和进食量。饲养30 d后处死小鼠，测量一系列肥胖指标，计算Lee’s指数，脂肪指数和各脏器指数，脂肪组织HE染色。结果表明，山腊梅醇提物和挥发油对小鼠脂肪的合成有干预作用，并且其可能机制是抑制脂肪合酶的活性，减少脂肪堆积。醇提高剂量组和挥发油组的Lee’s指数均显著低于正常组（分别下降了7.19%、6.96%），总脂肪指数有下降趋势（分别下降了42.04%、36.73%），并显著降低血清甘油三酯（分别下降41.34%、32.96%）和总胆固醇（分别下降20.83%、14.58%）含量。此外，脏器指数的结果表明山腊梅叶醇提取物对小鼠无明显毒性。

The in vitro screening experiment found that Chimonanthus nitens Oliv. leaf alcohol extract and volatile oil significantly inhibited the activity of fatty acid synthase (FAS). During the in vivo experiments, mice were divided into normal group, intake-control group, high-dose of alcohol extract group, low-dose of alcohol extract group and volatile oil group whose weight and food intake were recorded every two days. The mice were killed after 30 days and a series of obesity indicators were measured to calculate Lee’s index, fat index and index of each viscera. The fat in mice was studied through hematoxylin-esoinstaining. Results indicated that Chimonanthus nitens Oliv. leaf extract affected the synthesis of body fat, which probably due to the inhibition of FAS and interference in the accumulation of fat. High-dose of alcohol extract group and volatile oil group greatly decreased by 7.19% and 6.96% respectively compared with normal group, fat index decreased by 42.04% and 36.73% respectively, TG decreased by 41.34% and 32.96% respectively, and TC decreased by 20.83% and 14.58% respectively. Moreover, the visceral index showed that the extracts had no significant toxic effects to the mice.

国家自然科学基金资助项目（81273523）