本文建立了虎杖中五种活性成分：虎杖苷、白藜芦醇、大黄素-8-O-β-D-葡萄糖苷、大黄素、大黄素甲醚的高效液相色谱分析方法。色谱柱为Agilent Zorbax SB C18 柱（4.6×250 mm，5 μm），流动相为乙腈（A）和水（B），梯度洗脱程序为：0~40 min，15.0~50.0% A；40~60 min，50.0~90.0% A。柱温为40 ℃，检测波长为290 nm，流速为1.0 mL/min。五种成分线性良好（R>0.999），保留时间相对标准偏差（RSD）介于0.14~1.67%，峰面积RSD介于0.96~2.96%，加标回收率介于96.8~104.7%。优化了虎杖的提取条件，最佳条件为60%乙醇超声提取30 min。对10批虎杖样品分析结果表明：虎杖苷和大黄素-8-O-β-D-葡萄苷是虎杖乙醇提取物中两种含量最高的活性成分，含量范围分别为9.80~20.42 mg/g和5.56~24.45 mg/g。白藜芦醇和大黄素的含量范围分别为1.07~5.94 mg/g和3.79~17.85 mg/g，而大黄素甲醚含量为0.57~1.69 mg/g。该高效液相色谱法重复性好、精密度高，可用于虎杖中五种活性成分的同时测定。

Five bioactive compounds namely polydatin, resveratrol, emodin-8-O-β-D-glucoside, emodin and physcion in Polygonum cuspidatum were determined by HPLC. An Agilent Zorbax SB C18 column (250 mm×4.6 mm i.d., 5 μm) was used. The mobile phase consisted of acetonitrile (A) and water (B) with linear gradient program of 15.0~50.0% (A) in 0~40 min and 50.0~90.0% (A) in 40~60 min. The column temperature, detection wavelength and flow rate were set at 40 ℃, 290 nm and 1.0 mL/min respectively. The calibration curve of each analyte was constructed under six concentrations. The correlation coefficients were all more than 0.999, and the RSD of tR ranged from 0.14% to 1.67%, while RSD of the peak area was from 0.96% to 2.96%. The spike recovery of the five analytes was 96.8~104.7%. The extraction condition was optimized, and ultrasonic extraction with 60% ethanol for 30 min was chosen. The method was successfully applied for analysis of ten Polygonum cuspidatum samples. The results showed that polydatin and emodin-8-O-β-D-glucoside were the two most dominant constituents in Polygonum cuspidatum with contents ranges of 9.80~20.42 mg/g and 5.56~24.45 mg/g, respectively. The method has good repeatability and high precision, and can be used for simultaneous analysis of the five constitutes in Polygonum cuspidatum.

江西省自然科学基金资助项目（20122BAB214005）；江西农业大学青年基金资助项目（QN201108）