鮰鱼皮为斑点叉尾鮰鱼片加工的主要副产物，利用鮰鱼皮明胶制备降血压肽（ACE抑制肽）可为鮰鱼皮的高值化利用提供参考。本文通过对原发性高血压大鼠（SHR）进行一次性及长期给药（28 d）实验，研究鮰鱼皮明胶ACE抑制肽（Mr<3000 Da）的降血压作用，并测定大鼠血清和肺组织中的ACE活性和Ang II含量。实验结果表明：一次性给药和长期灌胃给药鮰鱼皮明胶ACE抑制肽均对SHR大鼠有显著降血压作用，降血压效果呈剂量依赖性；高剂量组SHR大鼠灌胃给药2 h后，其收缩压由206 mmHg降至159 mmHg；高剂量组SHR大鼠经长期灌胃给药10 d后，血压一直保持在155 mmHg左右；同时，鮰鱼皮明胶ACE抑制肽对正常血压大鼠的降血压作用不显著。鮰鱼皮明胶ACE抑制肽对SHR大鼠血清和肺组织中的ACE活性有显著的抑制作用，从而使大鼠血清和肺组织中Ang II含量显著降低，而对正常血压的SD大鼠的血清和肺组织中ACE活性和Ang II含量影响不显著。

Fish skin is the main byproduct in channel catfish filet processing industrial. Preparation antihypertensive peptides using channel catfish skin gelatin can promote its utility value. Spontaneously hypertensive rats (SHR) and normal SD rats were used to assess the antihypertensive effects of the ACE inhibitory peptides under optimized condition in vivo. The ACE activity and Ang II contents in the serum and lung tissue of SHR were measured. The blood pressure of SHR was decreased significantly after one-time dosing experiment and long-term dosing study (28 days), and the antihypertensive effects were dose-dependent. The blood pressure of SHR in high dose group decreased from 206 mmHg to 159 mmHg after 2 h oral administration and remained around 155 mmHg after long-term oral administration for 10 days. Moreover, the ACE inhibitory peptides had no significant effect on normal SD rats. The ACE inhibitory peptide from channel catfish skin gelatin inhibited the ACE activity, and decreased the Ang II contents in serum and lung tissue of SHR. While there was no significant effect on ACE activity and Ang II content in serum and hung tissue for normal SD rats.

国家自然科学基金资助项目（31000832）；国家农业科技成果转化资金项目（2012GB2C300202）