研究菝葜水提物对高脂饮食诱导肥胖小鼠体重和脂肪代谢的影响。50只ICR雌性小鼠随机分成5组，分别喂养基础饲料，高脂饲料和添加了不同剂量菝葜水提物的高脂饲料，定期测定体重和摄食量，投喂8周后测定小鼠体重、肝脏和腹腔内脂肪组织（IPAT）重量，分析血清中生化指标和肝脏中脂肪代谢相关酶的活性。与高脂组相比，低、中和高剂量的菝葜水提物能显著的控制小鼠体重增加、IPAT重量和血清中GLU和TG浓度，体重增加分别减少了31.97%、38.31%和36.90%，IPAT重量分别减少41.34%、48.58%和43.41%，GLU浓度分别降低了28.50%、17.15%和15.10%，TG浓度分别降低35.86%、29.31%、46.90%；肝脏中ACO的活性分别提升了25.49%、29.41%和26.47%，中、高剂量组AMPK活性显著提升分别提升了29.17%、33.33%。以上结果表明，菝葜水提物抑制小鼠体内脂肪沉积和减少体重增加主要是通过提升肝脏中脂肪氧化酶的活性，促进脂肪酸β-氧化来实现，对脂肪合成进程无明显影响。

Fifty female ICR mice were randomly assigned to five groups, individually fed on commercial diets，high-fat diet, high-fat diet with low, middle, high level of water extraction from Smilax china (WESC) for 8 weeks,. Body weight and food intake were inspected regularly during this period; after eight weeks, the weights of body, liver and intraperitoneal adipose tissue (IPAT) were measured, and the serum index and the activities of liver lipid metabolism related enzymes in mice were analyzed. Compared with the high-fat group, low, middle and high doses of WESC significantly decreased body weight gain, IPAT weight and the concentrations of GLU and TC in serum. The body weight gain decreased by 31.97%, 38.31% and 36.90%, respectively. IPAT weight decreased by 41.34%, 48.58% and 43.41%, respectively. The concentration of GLU reduced by 28.50%, 17.15% and 15.10%, respectively, and the TG concentration reduced by 35.86%, 29.31% and 46.90%, respectively. The activity of ACO in liver were increased by 25.49%, 29.41% and 26.47%, respectively. And the middle and high doses of WESC improved the activity of AMPK by 29.17% and 33.33%, respectively. The above results showed that the WESC suppressed fat accumulation and decreased the weight gain in mice, which was mainly due to increase of the activity of fat oxidation enzyme in liver, promotion of the fatty acid β-oxidation. No obvious influence was observed on fat synthesis.

国家自然基金资助项目（31160320）