生物被膜的形成给牛奶保藏和保鲜带来极大的隐患,探讨牛奶腐败过程中生物被膜形成特性以及寻求有效的抑制生物被膜的方法具有重要意义。本实验通过微孔板法模拟牛奶腐败过程，研究食源性金黄色葡萄球菌、肠炎沙门氏菌和大肠杆菌在牛奶中生物被膜形成能力、生物被膜中活菌数目与种间群体感应信号分AI-2活力三者之间的关系。随后利用AI-2抑制剂呋喃酮，检测三种细菌生物被膜形成能力及AI-2活力变化。最后证明，三种细菌生物被膜形成能力、生物被膜中活菌数目以及AI-2活力顺序均是金黄色葡萄球菌>大肠杆菌>沙门氏菌。添加呋喃酮之后，三种细菌的生物被膜形成能力以及AI-2活力均下降，其中金黄色葡萄球菌的生物被膜形成能力下降为原来的25%。因此表明，此三种细菌生物被膜形成能力的大小可能是是通过调节生物被膜中AI-2的活力实现的。

Bacterial biofilm brought great threat in milk preservation. Thus it is very important to research the formation features of biofilm during milk corruption and find an effect way to eliminate the biofilm. The relationship between the biofilm formation ability, the viable count in the biofilm and the AI-2 activity of Staphylococcus aureus, Salmonella and Escherichia coli in the process of milk spoilage were investigated by the method of microtitreplate. The change of biofilm and AI-2 activity after the addition of furanone was tested. Then the gradient concentrations of furanone indicated the relevance between the furanone and the quantity of bateria in the biofilm. The results showed that the sequence of three bacteria for biofilm formation ability, the viable count in the biofilm and the AI-2 activity was Staphylococcus aureus＞Escherichia coli＞Salmonella. Besides, the biofilm formation ability and AI-2 activity both declined after adding furanone, and the biofilm formation ability of Staphylococcus aureus reduced to 25%. Thus the results indicated that the ability of biofilm formation might be regulated by the AI-2 activity which was secreted by the viable isolates in the biofilm.

国家自然科学基金(31301480)；广东省211重点学科广东工业大学轻工化工学院创新人才项目；广东省大学生创新实验项目(2010044)