以脱脂银杏叶粉为原料，采用70%乙醇浸提法提取银杏叶黄酮，研究大孔树脂纯化银杏叶黄酮的工艺条件。以吸附率和解吸率为指标，考察了AB-8、D101、HPD-100 3种大孔树脂对银杏叶黄酮的吸附解吸性能，筛选出适合银杏叶黄酮分离纯化的树脂为AB-8型大孔树脂。结合静态与动态吸附解吸试验，得出AB-8大孔树脂分离纯化银杏叶黄酮的最佳工艺：将银杏叶黄酮提取原液稀释1.5倍（浓度为0.94 mg/mL）、调pH至4.85作为上样液，以1.5 BV/h的流速上样吸附，上样量200 mL，之后采用pH 4.95的80%乙醇作为洗脱剂，以2~2.5 BV/h的流速进行洗脱，洗脱剂用量约50 mL。在此纯化条件下所得银杏叶黄酮含量为26.16%，较纯化前提高了3.2倍。该纯化工艺条件科学合理，可有效用于银杏叶黄酮的分离富集，提高银杏叶提取物中的黄酮含量。

The flavones was extracted with 70% ethanol from degreased powder of Ginkgo biloba leaves (GBLs), and technical conditions of macroporous resins for the purification of flavones from GBLs were studied. The performances of adsorption-desorption of three resins AB-8, D101 and HPD-100 were investigated by using adsorption and desorption rate as indexes. And the AB-8 was considered to be the optimum resin to purify the flavones of GBLs due to its highest adsorption-desorption properties. Combination of static and dynamic experiments of resin adsorption-desorption, the preferable technical conditions of flavones of GBLs by AB-8 resin were obtained. The results were as follows: dilution of extracted solution 1.5 times (concentration of diluted sample 0.094 mg/mL), pH 4.85, loading velocity 1.5 BV/h, sample volume 200 mL, pH 4.95 eluant for desorption and 80% alcohol, desorption velocity 2~2.5 BV/h and eluant volume 50 mL. The flavones content of GBLs gained under these conditions was 26.16%, and 3.2 times more than that of the original sample. The technical condition is scientific and reasonable that can be used to separate and purify flavones of GBLs and improve the content of flavones in GBLs extracts.

江苏省普通高校研究生科研创新计划项目（CXZZ12_0544）；江苏省科技支撑（农业）项目（BE2011381）；国家林业局林业公益性行业科研专项项目（201004015）；江苏高校优势学科建设工程资助项目（PAPD）