建立高效液相转换波长法，测定金银花叶粗分物中绿原酸、木犀草素和木犀草苷的含量，绿原酸占水相原液的含量为2.05%，木犀草素、木犀草素在金银花叶粗提物EA相中的含量分别为1.12%、0.57%。以抗坏血酸(Vc)为阳性对照的，用DPPH?法、FRAP法、ORAC法三种体外抗氧化方法来测定和评价金银花叶有效成分的抗氧化作用。结果表明：金银花叶粗提物、粗分物、绿原酸.、木犀草素及木犀草苷均具有良好的抗氧化性，其中木犀草素的抗氧化能力最强，其IC50值为0.01764 mg/mL，还原能力1751.8±13.9 mmol/L，ORAC值23817.44 μmol Trolox/g。其次为粗分30%乙醇洗脱相，其IC50值为0.02548 mg/mL，还原能力933.8±11.6 mmol/L，ORAC值10557.97 μmol Trolox/g，且各物质随着抗氧化剂浓度及纯度的增加，抗氧化性逐渐增强。因此对金银花叶有效成分在抗氧化方面的应用具有广阔的开发利用前景，可作为新型的高效无毒的抗氧化剂。

A wavelength conversion method was established to determine chlorogenic acid, luteolin and luteoloside in Lonicera japonica leaves. The content of chlorogenic acid in aqueous phase was 2.05%, while the content of luteolin and luteoloside in EA phase were 1.12% and 0.57%, respectively. The antioxidative activity of effective components in Lonicera japonica leaves was evaluated by DPPH?, FRAP and ORAC with Vc as reference substance. The results showed that crude extraction, crude fraction, chlorogenic acid, luteolin and luteoloside in Lonicera japonica leaves all had great antioxidative activity. The strongest antioxidant capacity substance was luteolin, its IC50 value, reducing capacity and ORAC value were 0.01764 mg/mL, 1751.8±13.9 mmol/L and 23817.44 μmol Trolox/g, respectively. The crude fraction eluted by 30% ethanol also showed high antioxidative acitivity, its IC50 value, reduction capability and ORAC value were 0.02548 mg/mL, 933.8±11.6 mmol/L, and 10557.97 μmol Trolox/g, respectively. The antioxidative activity of each constituent increased with the increase of concentration and purity of the antioxidant. Therefore, there is broad development and utilization prospect of the antioxidant activity constituents of Lonicera Japonica leaves as a new type efficient non-toxic antioxidant.

广州市科技计划项目资助（2012J5100024）