采用气相熏蒸法研究了松针油、香茅油、依兰油、康酿克油、山苍子油、莪术油、鼠尾草油、沉香木油、艾叶油、迷迭香油十种植物精油对铜绿假单胞菌的气相抗菌活性，通过测定各种植物精油的最低抑菌浓度比较其抗菌效果。实验结果表明：山苍子精油和香茅精油的抑菌效果最好，其最低抑菌浓度均为250 μL/L；鼠尾草油和依兰油的最低抑菌浓度均为500 μL/L；艾叶油、莪术油和迷迭香油的最低抑菌浓度均为1000 μL/L；松针油、康酿克油和沉香木油未表现明显抗菌活性。山苍子油和香茅油以2:3和3:2进行复配时表现出显著协同增效效果，复配物空间体积浓度为100 μL/L时就能完全抑制铜绿假单胞菌的生长。不同溶剂对复配精油抗菌活性有所影响，以冰乙酸为溶剂抗菌效果最好，乙醇其次，甘油和丙二醇稍差。

The gas phase fumigation was used to study the vapor antibacterial activity of ten plant essential oils, namely pine needle oil, citronella oil, Ylang oil, Kang Niangke oil, Litsea cubeba oil, curcuma zedoary oil, sage oil, agarwood oil, mugwort oil, rosemary oil, against Pseudomonas aeruginosa. Antibacterial effects were compared by measuring the minimum inhibitory concentration (MIC). Results showed that Litsea cubeba oil and citronella oil presented the best inhibitory effects against Pseudomonas aeruginosa, the MIC of which were 250 μL/L, followed by sage oil and ylang oil with MIC of 500 μL/L. The MIC of mugwort oil, curcuma zedoary oil and rosemary oil were 1000 μL/L. Pine needle oil, Kang Niangke oil and agarwood oil showed no obvious antibacterial activities. When the compound ratio of Litsea cubeba oil and citronella oil was 2:3 or 3:2, they appeared significant synergistic effects. When the space volume concentration was 100 μL/L, the compound completely inhibited the growth of Pseudomonas aeruginosa. Different solvents had different antimicrobial activities on the compound essential oils, among which ice acetic acid had the best effect, followed by ethanol.

广东省部产学研合作专项资金(2012A090300010，2012B091100477，2011A090200004)