高血糖和高血脂已严重威胁人类健康，本研究探讨平卧菊三七（GPM）对小鼠血糖和血脂的影响，并通过分子生物学手段阐明其作用机制。将40只昆明小鼠随机分成4组，即对照组、2%、4%和8% GPM，喂养小鼠12周，称量小鼠体重和脏器，测定血中的生化指标和肝脏及粪中脂类含量，用qRT-PCR及蛋白印迹等方法分析肝脏mRNA和蛋白表达的变化。结果显示GPM对小鼠体重增加和脏器重量没有明显变化。4%以上GPM与对照组相比显著降低血中的甘油三酯（TG）、总胆固醇、低密度脂蛋白的胆固醇和血糖及肝脏中TG的浓度。而粪中的TG却明显增加。8% GPM明显抑制羟甲基戊二酰CoA还原酶（HMGCR）的活力和mRNA及蛋白表达水平，而Glut4的mRNA和蛋白表达明显增加。以上结果表明GPM通过调节HMGCR和Glut4的mRNA及蛋白表达，和抑制肠道对TG吸收，达到降糖降脂作用。

Hyperglycemia and hyperlipidemia have become strong threat on human health. This study investigated hypoglycemia and hypolipidemia effects of Gynura procumbens on mice, and to elucidate its mechanism by molecular biology methods. Forty Kunming mice were randomly divided into 4 groups: control, 2%, 4% and 8% Gynura procumbens fed for 12 weeks. Body and organs were weighed, and serum biochemical index and lipids levels in the liver and the feces were measured. The changes of mRNA and protein expression levels were analyzed by qRT-PCR and western blotting, respectively. Gynura procumbens had no significantly influence on body and organs weight in mice. Concentrations of triglycerides (TG) in blood, total cholesterols, low density lipoprotein (LDL) cholesterols, glucose in serum, and the hepatic TG level were remarkably decreased by feeding 4% GPM compared with control, while feces TG level obviously increased. The enzyme activity, mRNA and protein expression levels of HMG-CoA reductase (HMGCR) were significantly reduced by feeding 8% GPM, while mRNA and protein expression of Glut4 were up-regulated considerably by Gynura procumbens. The results suggested that Gynura procumbens had hypoglycemic and hypolipidemic efforts through regulating protein and mRNA expression of HMGCR and Glut4, and inhibiting TG absorption in intestinal tract.

国家自然基金资助项目（31160320）