本研究对市售的虫茶进行TCA8113人舌鳞癌细胞体外抗癌效果和体内抗转移效果评价。通过MTT试验、DAPI荧光染色分析和RT-PCR分析说明其体外抗癌效果，并通过建立动物模型分析其体内抗肿瘤转移效果。通过用50 μg/mL、100 μg/mL和200 μg/mL三种不同浓度的虫茶对TCA8113人舌鳞癌细胞体外增殖的抑制效果的研究表明，200 μg/mL浓度的虫茶(81%)表现出对该癌细胞有最强的生长抑制效果。通过用以上三种不同浓度的虫茶对该癌细胞的mRNA表达影响的研究表明，200 μg/mL浓度的虫茶比100 μg/mL和50 μg/mL虫茶具有更强的细胞诱导凋亡效果，而且随着虫茶浓度增加，Bax、caspase-3和caspase-9的mRNA表达增强，而Bcl-2表达减弱。通过BALB/c小鼠肿瘤转移的研究表明，虫茶也能抑制由26-M3.1结肠癌细胞诱导的肿瘤转移。对虫茶进行了体外抗癌效能和动物体内抗肿瘤转移的比较实验，虫茶是一种具有抗癌效果的功能性食品。

In this study, a sandy tea product was purchased for the evaluation of in vitro anticancer effect on TCA8113 human tongue squamous carcinoma cells and in vivo anti-metastatic effect．By using MTT assay, apoptosis analysis by DAPI staining and RT-PCR assay, and western blot assay were employed to check the in vitro anticancer effect and in vivo anti-metastatic effect of Sandy tea at different concentration (50, 100 and 200 μg/mL) using animal models. Results showed that 200 μg/mL of sandy tea (81%) had the best inhibitory effect and apoptosis-inducing activity on TCA8113 human tongue squamous carcinoma cells compared to those samples at other concentrations.With increasing the tea concentrations, mRNA expressions of Bax, caspase-3 and caspase-9 was up-regulated, but Bcl-2 expression was down-regulated. BALB/c tumor anti-metastasis test showed that sandy tea inhibited in vivo tumor metastasis induced by colon 26-M3.1 cells in BALB/c mice. These results suggest that sandy tea had potential as an anticancer functional food.