从鸡骨草中分离纯化得到中性多糖ACPa和酸性多糖ACPb两种组分，通过高效凝胶渗透色谱（GPC）法测定了其相对分子质量和HPLC-ELSD法分析了其单糖组成同时考查了它们的红外光谱特征，并且采用细胞模型抗氧化能力（cellular antioxidant activity, CAA）评价法对其进行了抗氧化活性评价。结果表明：ACPa和ACPb均具有典型的多糖红外吸收峰，ACPa的糖苷键以α-吡喃型为主，ACPb的糖苷键包含型α-和β-吡喃型；ACPa和ACPb重均相对分子质量分别为1.24×105 Da和2.15×105 Da；ACPa是一种葡聚糖，ACP为杂多糖，含有鼠李糖、阿拉伯糖、半乳糖和葡萄糖，摩尔比为1:3.4:3.6:10.9；在一定浓度范围内，ACPa和ACPb的抗氧化作用随着浓度的增加而增强，当ACPa和ACPb的质量浓度分别为6.25 μg/mL和100.00 μg/mL时，CAA值达到20，但在此基础上继续增加多糖浓度，CAA值并未随浓度的增加而显著性增加。

Row polysaccharide (ACP) was extracted from air part of Abrus cantoniensis Hance by ethanol precipitation and further purified by DEAE anion exchange chromatography to obtain fractions ACPa and ACPb, for investigation of their antioxidant activity and preliminary characterization. Weight-average molar mass (MW) and monosaccharide composition of the polysaccharide were determined by gel permeation chromatography (GPC and High-performance liquid chromatography with evaporative light-scattering detection (HPLC-ELSD), respectively. A cellular antioxidant activity (CAA) assay was used to quantify the antioxidant activity of purified fractions by conducting in human liver carcinoma HepG2 cells. The results showed that weight-average molar mass (MW) of ACPa and ACPb were 1.24×105 Da and 2.15×105 Da, respectively. Infrared (IR) spectra analysis demonstrated that both of them had the typical characteristic absorption of polysaccharides, ACPa only contained glucose, while ACPb was a heteropolysaccharde, composed of rhamnose, arabinose, galactose and glucose in a molar ratio of 1:3.4:3.6:10.9. Moreover, cellular antioxidant activity (CAA) assay suggested a positive relationship between antioxidant effect and dose of ACPa and ACPb. In a certain concentration range，antioxidant effect of ACPa and ACPb increased with increasing their concentration. When concentration of ACPa and ACPb were 6.25 μg/mL and 100.00 μg/mL, respectively, CAA value reached 20. Further increasing their dose showed little effects on the antioxidant effects.