[关键词]
[摘要]
为研究金黄色葡萄球菌活的非可培养（VBNC）状态的复苏问题，采取温度、盐度和酸度3个因素复合诱导制备VBNC菌，尝试四种复苏方法，并以荧光定量PCR和PMA-qPCR技术结合的方法进行检测。结果证明：8%无菌Tween80可以使VBNC状态金黄色葡萄球菌48 h后复苏，同时PMA-qPCR能够有效检出VBNC状态金黄色葡萄球菌，克服传统平板计数法对于VBNC菌的漏检。
[Key word]
[Abstract]
In order to study the resuscitation of Staphylococcus aureus in the viable but non-culturable state (VBNC), three factors (temperature, salt concentrations and pH) were investigated to induce VBNC Staphylococcus aureus. Four different resuscitation methods were attempted and a new detection method using qPCR and PMA in combination with qPCR was established to detect Staphyloccocus aureus. The results showed that the VBNC samples could resuscitate in 48 h after disposed in 8% Tween80. In addition, PMA-qPCR was an effective method for detection of VBNC Staphyloccocus aureus, which efficiently overcome the shortcomings of plate counts.
[中图分类号]
[基金项目]
国家自然科学基金青年科学基金项目资助课题(31101279)；中央高校基本科研业务费(2011ZM0101)；高等学校博士学科点专项科研基金新教师类资助课题（20110172120034）；广东省省部产学研结合项目（2012B091100113）
