本研究比较了超声波法（UP）、超声波结合纤维素酶法（UCP）、超声波结合胰酶法（UPP）、传统热水提取法（TP）四种方法提取灵芝多糖的性质区别。结果表明采用超声波结合胰酶法提取多糖得率最高，可达4.93%，比传统水提法的多糖得率高1.58倍。相比传统水提法所得多糖的分子量，超声处理可显著降低多糖分子量，超声结合酶法提取对多糖的降解作用更加显著，其中胰酶效果最为明显，可将多糖分子量降至5 kDa以下。此外，采用超声波结合胰酶法提取的多糖也具有较好的抗氧化活性，其还原力在浓度1.0 mg/mL时为1.408，羟自由基清除能力在浓度2.0 mg/mL时为50.08%，ABTS+清除能力在浓度1.0 mg/mL时为82.5%，氧自由基吸收能力（ORAC）值可达2484.14 μmol Trolox/g。

Different extraction methods, including ultrasound extraction (UP), ultrasonic wave complex cellulose (UCP), ultrasonic wave complex pancreatin (UPP) and traditional hot water extraction (TP), were used to prepare Ganoderma Lucidum polysaccharide and the characteristics of the extracted polysaccharides were compared. Results showed that the extraction yield of polysaccharides by UPP reached 4.93%, 1.58 times higher than that by TP. The molecular weight of polysaccharides by UP was smaller than that by TP. The lowest molecular weight (<5 kDa) was found when UPP was employed. Moreover, the polysaccharides by UPP exhibited the highest reducing power (1.408 at 1.0 mg/mL), hydroxyl radical scavenging ability (50.08% at 2.0 mg/mL), ABTS+scavenging ability (82.5% at 1.0 mg/mL) and the oxygen radical absorbance capacity (2484.14 μmol Trolox/g).

国家自然科学基金（31101222和31101221）；SRP项目《生物活性多糖的分离纯化及功效研究》；广州地区分析测试基金（201208）