[关键词]
[摘要]
建立同时测定小根蒜中尿苷、鸟苷和腺苷含量的反相高效液相色谱(RP-HPLC)梯度洗脱法。采用ZORBAX-SB-C18色谱柱(150 mm×4.6 mm, 5 μm);以水-甲醇为流动相,梯度洗脱,流速为0.6 mL/min,柱温为25 ℃;检测波长:260 nm。尿苷在0.0784~0.784 μg范围内线性关系良好, 线性相关系数r=0.9995;鸟苷在0.0798~0.798 μg范围内线性关系良好,线性相关系数r=0.9995;腺苷在0.0852~0.852 μg范围内线性关系良好,线性相关系数r=0.9994。该法操作简便,方法可靠,重现性好,可以作为小根蒜的质量控制方法之一。
[Key word]
[Abstract]
A new method for determination of uridine, guanosine and adenosine contents in Allium macrostemon bunge were established by using RP-HPLC gradient elution. The extracts were separated on a ZORBAX-SB-C18 analytical column (4.60 mm×250 mm, 5 μm) with CH3OH-H2O as mobile phase at 260 nm. The flow rate was 0.6 mL?min- 1and the column temperature was 25 ℃. The linear ranges of uridine,guanosine and adenosine were 0.0784~0.784 μg(r=0.9995), 0.0798~0.798 μg(r=0.9995)and 0.0852~0.852 μg (r=0.9994), respectively. This method showed high reproducibility and can be used for quality control of the Allium macrostemon bunge.
[中图分类号]
[基金项目]
贵州省科技创新人才团队项目(合同号:黔科合人才团队(2010)4011号)