本文通过测定皱纹盘鲍的冻结曲线获得了生态冰温区，开展了皱纹盘鲍生态冰温保活工艺的研究。针对不同温度、不同降温模式、净化和充氧条件等开展皱纹盘鲍保活对比实验，寻求最适宜的生态冰温保活工艺。实验结果表明生态冰温区（-1.5 ℃~0 ℃）保活条件下皱纹盘鲍的存活率最佳，梯度降温和保活时的冷海水喷淋可显著提高皱纹盘鲍的存活率，此外净化和充氧可明显提高皱纹盘鲍的存活率。本文获得的皱纹盘鲍最佳保活工艺为：对皱纹盘鲍进行净化，采用充氧冷海水喷淋皱纹盘鲍将其梯度降温至生态冰温区、在保活阶段采用充氧冷海水喷淋为皱纹盘鲍提供氧气和潮湿的环境。

The ice temperature zone of hannai was obtained by measuring its freezing curve and then the technologies for keeping Halitotis discus hannai alive at ecological ice temperature were investigated. Results show that the survival rate of Haliotis discus hannai is the highest while it was kept alive at ecological ice temperature (-1.5 ℃~0 ℃). The treatments by gradient freezing, refrigerated seawater spray, cleaning and oxygen supplement were good for Haliotis discus hannai’s survival rate improvement. The best processing conditions for keeping alive were: cleaning Haliotis discus hannai ,then gradient freezing it to ecological ice temperature by spraying refrigerated seawater with oxygen supplely, and continuing spraying refrigerated seawater and supplying oxygen and moisture environment during the keeping-alive stage. Key words: Haliotis discus hannai; ecological ice temperature; keeping alive; refrigerated seawater spraying

2010年农业部渔业装备与工程重点开放实验室开放课题项目（2010A08）；国家863科技计划项目（2011AA100803）；国家贝类产业技术体系资金资助（CARS-48-08B）