应用响应面法对超声与微波联合提取玉米须甾醇的工艺进行优化，建立了相应的回归模型。在单因素试验的基础上，考察超声时间、超声波功率、液料比和微波时间对玉米须甾醇得率的影响。通过中心组合试验优化获得最佳工艺条件为：超声时间55 min，超声波功率200 W，料液比1:40 (g/mL)，微波时间16 min，通过五次验证试验，玉米须甾醇的得率为0.76%，与理论预测值基本符合，为玉米须甾醇的开发提供理论依据。

In order to optimize ultrasonic and microwave assisted extraction process for phytosterol from maize silk, effects of ultrasonic time, ultrasonic power, ratio of solid-liquid and microwave time on phytosterol extraction rate from corn stigma were evaluated. Based on single factor tests, Box-Behnken experimental Design was used and a regression model was established by Response Surface method. Results showed that optimum process conditions were as follows: ultrasonic time 55.00 min, ultrasonic power 200 W, solid-liquid rate 1:40 (g/mL) and microwave time 16 min. Under these conditions, 0.76% of phytosterol extraction rate was obtained. Tested and predicted values for phytosterol extraction rate were similar basically. The results contributed to the development of phytosterol from maize silk.

吉林省科技发展计划项目(20120717)；吉林大学基本科研业务费项目（200903263）