本实验采用混菌发酵技术，以信州灵芝和金针菇为出发株，探讨灵芝、金针菇混菌发酵高产锌元素的工艺条件，采用原子吸收分光度法测定胞内锌元素含量。结果表明：最佳培养基为2%山芋淀粉、2%马铃薯、1%蔗糖、2%玉米粉、1%黄豆粉；混菌发酵最佳培养条件为培养温度27 ℃，转速150 r/min，装液量100 mL/250 mL，培养时间8 d；在最佳条件下，混菌发酵胞内锌元素含量为45.6 mg/kg，比灵芝单独培养提高15.8%，比金针菇单独培养提高63.5%。说明混菌发酵更完全，对培养基中的营养物质吸收利用率高，菌丝体长势好，增加了胞内锌产量。

G.lucidum XinZhi and Flammulina velutipes were used as the original strains for mixing fermentation to discuss technology conditions of high-yield zinc. Quality of zinc from mycelium was determined by atomic absorption spectrum method. The results showed that the best medium was 2% starch, 2% potato, 1% sucrose, 2% corn meal, and 1% soybean meal. The optimal fermentation conditions were cultured time 8 days, temperature 27 ℃, shaking speed 150 r/min, and medium volume 100 mL/250mL. Quality of intracellular zinc was 45.6 mg/kg at above optimal conditions, 15.8% and 63.5% higher than that by G.lucidum XinZhi and Flammulina velutipes respectively. Therefore mixed fermentation had a more complete process, higher availability of nutrition, promising growth and more quality of intracellular zinc.

徐州市科技计划项目（XF11C009）