本文建立了HPLC测定茶饮料中阿斯巴甜含量的方法，采用色谱柱：THERMO C18（4.6×250 mm，5 μm），检测波长：210nm，流量：1.0 mL/min，进样量：20 μL，流动相：0.01 mol/L磷酸二氢钾-乙腈为85:15。结果表明：阿斯巴甜在0~50 μg/mL范围内呈现良好的线性关系，Y=52139X（0~50 μg/mL）（R=0.9994）。平均回收率100.3%，RSD=0.33%。本方法简单，快速、准确、重现性好，可以用作茶饮料中阿斯巴甜的含量测定。

To establish a high performance liquid chromatography (HPLC) method for determination content of Aspartame in tea beverages ,20 μL samples were tested on a thermo C18 column using 0.01 mol?L-1 potassium dihydrogen phosphate and acetonitrile (40:60) as mobile phase was with flow rate of 1.0 mL/min at 210nm.The results showed that Aspartame concentration had good liner relationship within the range of 0~50 μg/mL (Y=52139X (0~50 μg/mL, R=0.9994). The average recovery was 100.3% with RSD of 0.33%.The method was simple, fast, accurate, and precise, which could be used to determine content of aspartame in tea beverages.