本文以水酶法提取油茶籽油后产生的工艺水为原料，研究了微生物发酵法提取油茶皂素的工艺，得出较优的菌种组合为褶皱假丝酵母和米曲霉；较佳发酵条件为：总接种量8%，接种比例为2:3，发酵时间为4 d。按此优化条件进行三角瓶液态发酵，油茶籽工艺水经发酵后蛋白质含量降低了60.99%，多糖含量降低了54.02%，从工艺水中提取的油茶皂素的纯度从发酵前的36.45%提高到发酵后的58.74%，且油茶皂素产品的颜色由原来的黑褐色变为棕黄色。

The purification technique of camellia saponin by mixed microbial fermentation was studied via using oil Camellia seed wastewater as raw materials. The optimal results were achieved by Candida rugosa and Aspergillus oryzae. The optimal fermented condition were the total inoculation quantity 8%, the ratio of inoculation 2:3 and the fermentation time 4 days. By liquid fermentation tests under the above mentioned conditions, protein content was reduced by 60.99% and polysaccharide content was lowered by 54.02%. Purity of camellia saponin improved from 36.45% to 58.74% and the color of camellia saponin products was changed from dark brown to pale brown.

湖南省科技厅重大专项（04NK1008）