在10 L发酵罐中研究了间歇补料对小单孢菌发酵生产庆大霉素的影响，以期提高庆大霉素的产量，为建立生物制药工艺综合性实验奠定基础。在保持发酵罐pH恒定的基础上，以摇瓶发酵优化条件作为发酵罐初始发酵条件，分别采用不同的间歇补料方式，在发酵的初期、中期和后期流加糊化玉米淀粉，探索较优的碳源补料方式。结果表明，少量多次补料方式的发酵水平优于多量少次补料方式。在少量多次补料方式的基础上，增加溶氧量，不仅延长了产素期，而且发酵水平较初始发酵提高了87.3%。说明优化后的间歇补料方式不仅延长了发酵周期，而且提高了庆大霉素发酵产量。

In order to enhance gentamicin production and build up the foundation for comprehensive experiment in Bio-pharmaceutical Technology, the influence of fed-batch fermentation on the gentamicin production of Micromonospora in a 10 L fermentor was studied.The result showed that fermentation production under frequent small amounts feeding pattern was better than that under the feeding pattern with large amounts at once. Increasing the amount of dissolved oxygen under the frequent small amounts feeding pattern not only extended production phase, but also increased fermentation productivity to 87.3%. It was concluded that the optimized fed-batch pattern can not only extend fermentation period, but also improve the yield of gentamicin.

华南理工大学生物科学与工程学院，广东广州 510006