该研究为了获得碱法提取败酱草中不溶性膳食纤维的最适工艺参数，以温度、时间和碱浓度为试验因子，以不溶性膳食纤维产率为响应值，采用单因素试验和L9(34)正交试验进行优化试验。结果表明：碱法提取败酱草不溶性膳食纤维的最佳提取工艺为氢氧化钠浓度4%、处理温度40 ℃、处理时间1 h，在此条件下不溶性膳食纤维得率为89.46%，且无粗糙感，色泽良好。因此从败酱草中提取不溶性膳食纤维具备可行性。

Optimization of extraction conditions for preparing insoluble dietary fiber from the Herba Patriniae was studied by single factor method coupling with L9(34) orthogonal array design with three variables of temperature, time and alkali concentration. The yield of insoluble dietary fiber was used as evaluation index. The results indicated that the optimal conditions for preparation of insoluble dietary fiber were: alkali concentration 4%, temperature40 ℃ and time 1 h. Under the optimal conditions, the experimental yield of insoluble dietary fiber was 89.46% and the product showed no rough sense and fine color. Therefore, the extraction of insoluble dietary fiber from Herba Patriniae has great feasibility in the comprehensive utilization of Herba Patriniae.

辽宁省科技厅博士启动项目（20111146）