[关键词]
[摘要]
采用一种活性新琼寡糖作为免疫增强剂,通过在饲料中添加不同含量的新琼寡糖(分别为饲料重量的0.1%,0.5%,1%,1.5%和3%),以血清溶菌酶活力、血清补体活性及白细胞总数和白细胞吞噬率为判断依据,确定新琼寡糖对于鲍鱼非特异性免疫力的影响;同时用嗜水气单胞菌(Aeromonas hydrophila)对各组鲍鱼进行细菌感染试验,测定鲍鱼存活率,以确定新琼寡糖对鲍鱼抗病力的影响。结果表明:投喂新琼寡糖组的鲍鱼的血清溶菌酶活力、血清补体活性及血液白细胞总数和吞噬率均明显高于对照组,且新琼寡糖的最佳投喂量为饲料重量的0.5%~1%。细菌感染试验结果显示,在细菌感染72 h后,对照组鲍鱼的存活率最低,仅为42%,而新琼寡糖投喂组鲍鱼的存活率均达到58%以上,且最高可达到79%。
[Key word]
[Abstract]
In this study, the activity of neoagaro-oligosaccharides as a new immunostimulant was studied by adding different dosages of Neoagaro-oligosaccharides in the feed (0.1%, 0.5%, 1%, 1.5% and 3%, respectively). Then, determination of serum complement activity, lysozyme activity, phagocytic activity and the total number of leucocytes was carried out to decide the impact of neoagaro-oligosaccharides on abalone non-specific immunity. Besides, the abalone were injected peritoneally with Aeromonas hydrophila after fed 3 months for determine the effect of neoagaro-oligosaccharides on the disease resistance. The results showed that the serum complement activity, lysozyme activity, phagocytic activity and the total number of leucocytes of the groups of adding neoagaro-oligosaccharides in the feed were significantly higher than that of the control group. The best dosage of the Neoagaro-oligosaccharides in the feed was between 0.5% and 1%. The results of the bacterial infection test showed that the survival rate of neoagaro-oligosaccharides feeding group was significantly higher than that of the control group.
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[基金项目]
国家自然科学基金项目(40776091)