敌敌畏（DDVP）是养殖水体的常见污染物，为了评估敌敌畏对鱼类谷丙转氨酶（GTP）和谷草转氨酶（GOP）的影响，将草鱼暴露于含5~20 mg/L敌敌畏的水体中饲养10 d，测定肝脏和肾脏中GTP及GOP活性的变化。实验结果表明，DDVP对草鱼的96 h LC50为22.9 mg/L。亚致死浓度DDVP暴露可导致草鱼GTP和GOP活性发生变化。在所有受检组织中，GTP和GOP活性在暴露初期均受到不同程度的诱导，但随着DDVP浓度升高和暴露时间延长，酶活性呈下降趋势，提示DDVP暴露所引起的酶活性变化与暴露浓度和暴露时间有一定的相关性。此外, GTP和GOP在肝脏中胁迫明显，可以作为DDVP污染的生物标记。

Grass carps was exposed to an aquatic environment with DDVP concentration of 5~10 mg/L for 10 days. GTP and GOP activities in the kidney and liver of the fish were determined. The results showed that the LC50 of DDVP (96 h) to grass carp was 22.9 mg/L. The activities of GTP and GOP were determined in grass carp after exposure .The two enzymes in all tested tissues were induced in the initial exposure days, and then the enzyme activities decreased with increase of DDVP concentration and extension of exposure time, suggesting that the enzyme activities correlated to the DDVP concentration and the exposure time. GTP and GOP were sensitive to DDVP exposure in liver, which could be used as a more suitable biomarker for DDVP pollution.