本实验运用布鲁姆林式脉冲成形网络系统对副溶血性弧菌菌悬液进行处理，发现该系统对副溶血性弧菌有较强的灭菌效果，同时细胞毒性有所下降，且随电压升高而降低；而三型分泌系统相关的基因表达随电压升高而增强，表明脉冲高压电场对细菌病原性有一定的影响，可为液体食品灭菌、生物制品以及疫苗制备过程中抗原灭活提供一定的理论依据。

A Blumlein type pulse forming network system was developed for treating Vibrio parahaemolyticus suspension solution. The results showed a high disinfection ability of this high voltage pulse electric fields on Vibrio parahaemolyticus. Meanwhile, the cytotoxicity of the bacteria was reduced after the bacteria were treated by this system. The expression level of three type secretion system -related genes increased with the increase of voltage, indicating that high-voltage pulse electric fields affected the pathogenic of bacteria. This research could be a theoretical base for liquid food disinfection, bioproducts and vaccine inactivation.

衡阳市科技局（2011KJ19）