光谱分析的测定值回归方程包含测定的不确定度来源，采用方差分析计算合成不确定度时，标准曲线的斜率和截距为非独立因子，应加入两者组合的标准不确定度。经数学推导将合成不确定度的偏微分计算演变为函数计算。包含因子采用欧洲通用常数，适合我国高新仪器应用现状。

The factors in regression equation of measurement compose the sources of measurement uncertainty. The combined uncertainty and its components should be expressed in the form of standard uncertainty, using slope and intercept of the calibration equation as the independent parameters. Convenient function calculation is performed from partial derivative one by means of mathematical treatment. Coverage factor in expanded uncertainty, the commonly-used one in Europe, was adapt, which was suitable for the excellent instrument situation in China.