本文研究了以胡萝卜素降解物作为免疫诱导剂的H22全细胞免疫物对小鼠H22肝癌的抑制作用，通过用胡萝卜素降解物与H22细胞共培养后对小鼠进行免疫，同时设立模型组和空白组，试验期间，测定小鼠生长曲线、脏器指数、抑瘤率、巨噬细胞吞噬能力、NK细胞杀伤活性、免疫器官HE染色等。结果表明：胡萝卜素降解物对小鼠没有毒性作用，免疫组小鼠抑瘤率为93%、巨噬细胞吞噬指数和廓清指数分别为5.42±0.19、0.39±0.002，NK细胞杀伤活性为0.93，总体各个指标均与模型组小鼠呈显著性差异，说明H22全细胞免疫物能够增强小鼠的免疫能力，有效保护免疫器官。

The effects of H22 cells vaccine treated with carotene degradants as immunoadjuvant on the mice were studied in this paper. By immunising the mice with the coculture of H22 cells and carotene degradants, setting the model and the control, it was found that the carotene degradants had no toxic effects on the mice and the cancer inhibition rate of immue mice was 93%. Carbon particle clearance and the phagocytsis index of macrophages were 0.39±0.002 and 5.42±0.19, respectively. Cytotoxicity of NK cells was 0.93. All of the parameters had significant difference compared with those of the model. The research proved that H22 cells vaccine treated with carotene degradants can effectively protect immune organs.

国家自然科学基金（31000755）；科技部科技型中小企业技术创新基金（10C26211200196）